{"product_id":"biolegend-105827","title":"Biolegend, 105827, Brilliant Violet 421™ anti-mouse CD117 (c-Kit) Antibody, 125microl","description":"\u003cp\u003eCD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and\/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.\u003cbr\u003e\n125microl\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse bone marrow mast cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested SB - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.\u003cbr\u003e\nAdditional Product Notes: Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC) Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC) Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC) Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC) Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Tan J, et al. 2021. iScience. 24(8):102835. PubMed Kaltenbach L, et al. 2023. Nat Immunol. 24:915. PubMed Feng X, et al. 2023. Nat Commun. 14:3208. PubMed Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed Issa N, et al. 2023. Leukemia. :. PubMed Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed Bowers E, et al. 2018. Nat Med. 24:95. PubMed Liu L, et al. 2022. Cancers (Basel). 14:. PubMed Thiele K, et al. 2015. Am J Pathol. Available online 5 August 2015. PubMed Wu W, et al. 2014. Proc Natl Acad Sci U S A. 111:4221. PubMed Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed Emgård J, et al. 2018. Immunity. 143:419. PubMed Carpenter RS, et al. 2020. Nat Commun. 3.029166667. PubMed Tyrkalska SD, et al. 2019. Immunity. 51:50. PubMed Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed Wei Q, et al. 2020. Dev Cell. 53:503. PubMed Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed Schuler F, et al. 2017. Nat Commun. . 10.1038\/s41467-017-01850-4. PubMed Celik H, et al. 2018. Cancer Cell. 34:741. PubMed Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Gao X, et al. 2021. Nature. 589:591. PubMed Lefkopoulos S, et al. 2020. Immunity. 53(5):934-951.e9. PubMed Iwanami N, et al. 2020. iScience. 23:101260. PubMed Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed Boulch M, et al. 2021. Sci Immunol. 6:. PubMed Gross KM, et al. 2019. Cell Rep. 28:394. PubMed Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed Strattan E, et al. 2020. Frontiers in Immunology. 2.131944444. PubMed Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed Abdel Malik R, et al. 2017. Circ Res. 120:99. PubMed\u003cbr\u003e\nRRID: AB_10898120 (BioLegend Cat. No. 105827) AB_11204256 (BioLegend Cat. No. 105828)\u003cbr\u003e\nStructure: Ig superfamily, 145 kD\u003cbr\u003e\nDistribution: Hematopoietic stem cells, AML, mast cells\u003cbr\u003e\nFunction: Growth factor receptor, tyrosine kinase\u003cbr\u003e\nLigand\/Receptor: Stem Cell Factor (SCF)\u003cbr\u003e\nCell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells\u003cbr\u003e\nBiology Area: Immunology, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Galli SJ, et al. 1994. Adv. Immunol. 55:1. 3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759. 4. Besmer P, et al. 1986. Nature 320:415. 5. Witte ON. 1990. Cell 63:5.\u003cbr\u003e\nGene ID: 16590\u003cbr\u003e\nUniProt: View information about CD117 on UniProt.org\u003cbr\u003e\nClone: 2B8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: c-KIT, Stem Cell Factor Receptor (SCFR)\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003cbr\u003e\nQ: If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?\u003cbr\u003e\nA: It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.\u003cbr\u003e\nQ: Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?\u003cbr\u003e\nA: Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF\/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.\u003cbr\u003e\nQ: Are other fluorophores compatible with IBEX?\u003cbr\u003e\nA: Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.\u003cbr\u003e\nQ: The same antibody works in one tissue type but not another. What is happening?\u003cbr\u003e\nA: Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.\u003cbr\u003e\nQ: How can I be sure the staining I’m seeing in my tissue is real?\u003cbr\u003e\nA: In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866542133417,"sku":"105827","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-105827","provider":"Iright","version":"1.0","type":"link"}