{"product_id":"biolegend-106006","title":"Biolegend, 106006, PE anti-mouse CD123 Antibody, 100μg","description":"\u003cp\u003eCD123 is a 70 kD α chain subunit of the IL-3 receptor (IL-3R α). It is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL-3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either the common β chain (CDw131) or the IL-3R β chain (AIC2A) to form high-affinity heterodimeric IL-3 receptors. CDw131 can complex with the α subunits of the mouse IL-3R, IL-5R and GM-CSFR to form high-affinity receptors, while the IL-3 R β subunit is specific for IL-3 but binds with low affinity. IL-3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. The 5B11 antibody does not block binding of IL-3 to the high affinity IL-3 receptor.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: CTLL cells transfected with the mouse IL-3Rα chain\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106006) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106004) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16\/CD32, clone 93 (Cat. No. 101301\/101302), is recommended prior to immunofluorescent staining.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ichihara, et al. 1995. EMBO J. 14:939. (FC) Mueller DL, et al. 1994. J. Immunol. 153:3014. (FC) Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC)\u003cbr\u003e\nProduct Citations: Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed Shiozawa S, et al. 2022. iScience. 25:103537. PubMed Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed\u003cbr\u003e\nRRID: AB_2124403 (BioLegend Cat. No. 106005)\u003cbr\u003e\nStructure: Ig superfamily, associates with CDw131 (common β chain) or IL-3 receptor β chain, 70 kD\u003cbr\u003e\nDistribution: Hematopoietic progenitors, basophils, mast cells, megakaryocytes\u003cbr\u003e\nFunction: Proliferation, differentiation of hematopoietic cells\u003cbr\u003e\nLigand\/Receptor: IL-3\u003cbr\u003e\nCell Type: Basophils, Dendritic cells, Hematopoietic stem and progenitors, Mast cells, Megakaryocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Miyajima A, et al. 1993. Blood 82:1960. 3. Itoh N, et al. 1990. Science 247:324.\u003cbr\u003e\nGene ID: 16188\u003cbr\u003e\nUniProt: View information about CD123 on UniProt.org\u003cbr\u003e\nClone: 5B11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IL-3 Receptor α chain, IL-3Rα\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862447837353,"sku":"106006","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-106006","provider":"Iright","version":"1.0","type":"link"}