{"product_id":"biolegend-106309","title":"Biolegend, 106309, APC anti-mouse CD152 Antibody, 25μg","description":"\u003cp\u003eCD152, also known as CTLA-4 or Ly-56, is a 33 kD member of the immunoglobulin superfamily. It is expressed on activated T and B lymphocytes. CD152 is similar to CD28 in amino acid sequence, structure, and genomic organization and these two receptors share common B7 family counter-receptors (B7-1, B7-2). Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses. CD152 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: Mouse CTLA-4-mouse IgG2a fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: The UC10-4B9 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro stimulation, in vitro and in vivo blocking1-4 of ligand binding, and as ELISA capture antibody5. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16\/CD32, clone 93 (Cat. No. 101301\/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The Ultra LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 106327).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Walunas TL, et al. 1994. Immunity 1:405. (Block, IP) Cilio CM, et al. 1998. J. Exp. Med. 188:1239. (Block) Issazadeh S, et al. 1999. J. Immunol. 162:761. (Block) McCoy K, et al. 1997. J. Exp. Med. 186:183. (Block) Hsu HC, et al. 2007. J. Immunol. 178:5357. (ELISA Capture) Sugita S, et al. 2010. Invest. Ophthalmol. Vis. Sci. 51:5783. PubMed\u003cbr\u003e\nProduct Citations: Lehmkuhl P, et al. 2021. Cell Mol Immunol. 18:1677. PubMed Yang H, et al. 2023. Cell Death Differ. 30:560. PubMed Schmitt E, et al. 2013. J Immunol. 191:5640. PubMed Zhang P, et al. 2017. Int J Biol Macromol. 10.1016\/j.ijbiomac.2017.06.023. PubMed Lu SX, et al. 2021. Cell. . PubMed Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed Gubser C, et al. 2016. Sci Rep. 6: 25758. PubMed Wong HS, et al. 2021. Cell. . PubMed Sidwell T, et al. 2020. Nat Commun. 0.633333333. PubMed Emmerson A, et al. 2018. J Clin Invest. 128:3088. PubMed Espinosa JR, et al. 2018. Front Immunol. 9:1371. PubMed Yue X, et al. 2019. Nat Commun. 10:2011. PubMed Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed Studniberg SI, et al. 2022. Mol Syst Biol. 18:e10824. PubMed Koikawa K, et al. 2021. Cell. 184:4753. PubMed Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed Severance AL, et al. 2022. iScience. 25:104400. PubMed Takenori Inomata, Jing Hua, Antonio Di Zazzo 2016. Sci Rep. 6:39924. PubMed Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed Fedele C, et al. 2021. J Exp Med. 218: . PubMed Henkle TR, et al. 2021. Cancer Res. 81:4560. PubMed Long L, et al. 2021. Nature. 600:308. PubMed\u003cbr\u003e\nRRID: AB_2230158 (BioLegend Cat. No. 106309) AB_2087653 (BioLegend Cat. No. 106310)\u003cbr\u003e\nStructure: Ig superfamily, 33 kD\u003cbr\u003e\nDistribution: Activated T cells and B cells\u003cbr\u003e\nFunction: Negative regulator of T cell activation\u003cbr\u003e\nLigand\/Receptor: CD80 (B7-1), CD86 (B7-2)\u003cbr\u003e\nCell Type: B cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Allison JP, et al. 1995. Science 270:932. 3. Waterhouse P, et al. 1995. Science 270:985. 4. Linsley PS, et al. 1991. J. Exp. Med. 174:561.\u003cbr\u003e\nGene ID: 12477\u003cbr\u003e\nUniProt: View information about CD152 on UniProt.org\u003cbr\u003e\nClone: UC10-4B9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864066511017,"sku":"106309","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-106309","provider":"Iright","version":"1.0","type":"link"}