{"product_id":"biolegend-107205","title":"Biolegend, 107205, PE anti-mouse CD273 (B7-DC, PD-L2) Antibody, 50μg","description":"\u003cp\u003eB7-DC is also called programmed death ligand 2 (PDL2). It has recently been clustered as CD273. This ligand is a 42 kD member of the immunoglobulin receptor superfamily expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions. The TY25 antibody has been reported to be useful for blocking PD-1 mediated interactions.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse B7-DC transfected cell line\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1,6, blocking2,4,5 of PD-1 mediated interactions, and immunohistochemistry of acetone-fixed frozen sections3. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107221-107226).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Yamazaki T, et al. 2002. J. Immunol. 169:5538. (FC, IP, WB) Ansari MJI, et al. 2003. J. Exp. Med. 198:63. (Block) Salama AD, et al. 2003. J. Exp. Med. 198:71. (IHC) Matsumoto K, et al. 2004. J. Immunol. 172:2530. (FC, Block) Yamazaki T, et al. 2005. J. Immunol. 175:1586. (Block) Meng Q, et al. 2006. Invest Opthalmol Vis Sci. 47:444. (WB) PubMed del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed Murakami R, et al. 2013. PLoS One. 8:73270. PubMed\u003cbr\u003e\nProduct Citations: Ochiai S, et al. 2014. J Immunol. 193:2504. PubMed Lakins MA, et al. 2018. Nat Commun. 9:948. PubMed Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed Yang Z, et al. 2022. Cancer Discov. 12:1942. PubMed Yonemitsu K, et al. 2022. Sci Rep. 12:12007. PubMed Quatrini L, et al. 2018. Nat Immunol. 19:954. PubMed Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed Fu Y, et al. 2020. Sci Rep. 10:9027. PubMed Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed Abbott RK, et al. 2018. Immunity. 48:133. PubMed Jelinek D, et al. 2014. J Immunol. 192:3548. PubMed Pizzolla A, et al. 2016. PLoS One. 11: 0160407. PubMed Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed Montero-Barrera D, et al. 2015. BioMed Res Int. 2015 615865. PubMed Knox T, et al. 2019. Sci Rep. 9:6136. PubMed Nakagawa Y, et al. 2015. Immunol Lett. 167: 72-86. PubMed Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed\u003cbr\u003e\nRRID: AB_2299418 (BioLegend Cat. No. 107205) AB_2162011 (BioLegend Cat. No. 107206)\u003cbr\u003e\nStructure: B7 Immunoglobulin superfamily, 42 kD\u003cbr\u003e\nDistribution: Dendritic cells, liver, few transformed cell lines, subset of macrophages; not expressed on lymphoid tissues\u003cbr\u003e\nFunction: Binds to PD-1 and alternative receptor; ligation on DC stimulates, inhibits T cell responses via PD-1 binding, stimulates T cells via alternative receptor binding and promotes tumor immunity\u003cbr\u003e\nLigand\/Receptor: PD-1, uncharacterized receptor\u003cbr\u003e\nCell Type: Dendritic cells, Macrophages\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Shin T, et al. 2003. J. Exp. Med. 198:31. 2. Liu X, et al. 2003. J. Exp. Med. 197:1721. 3. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.\u003cbr\u003e\nGene ID: 58205\u003cbr\u003e\nUniProt: View information about CD273 on UniProt.org\u003cbr\u003e\nClone: TY25\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PD-L2, PDL2, B7DC, CD273\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864062709929,"sku":"107205","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-107205","provider":"Iright","version":"1.0","type":"link"}