{"product_id":"biolegend-108315","title":"Biolegend, 108315, PerCP\/Cyanine5.5 anti-mouse Ly-51 Antibody, 25μg","description":"\u003cp\u003eLy-51 is a 140 kD protein also known as 6C3\/BP-1. It is a homodimeric cell-surface glycoprotein with aminopeptidase A (APA) activity. Ly-51 is expressed on B cell progenitors, bone marrow stromal cell lines, thymic dendritic cells, and cortical epithelial cells. Ly-51 expression can be upregulated by IL-7 stimulation.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: C57BL\/6 mouse Pre-B lymphoma cell line (L1-2) plus Abelson murine leukemia virus-specific cytotoxic T-cell clones\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1,2 and immunohistochemical staining3-5, 7-8 of acetone-fixed frozen sections.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Pillemer E, et al. 1984. P. Natl. Acad. Sci. USA 81:4434. (IP) Hardy RR, et al. 1991. J. Exp. Med. 173:1213. (IP) Adkins B, et al. 1988. Immunogenetics 27:180. (IHC) Surh CD, et al. 1992. J. Exp. Med. 176:611. (IHC) Goverman J, et al. 1997. Immunol. Today 18:204. (IHC) Dumont-Lagace M, 2014. J. Immunol. 192:2219. PubMed Kvell K, et al. 2010. PLoS One 5:e10701. (FC, IHC) Griewank K, et al. 2007. Immunity 27:751. (FC, IHC)\u003cbr\u003e\nProduct Citations: Xing W, et al. 2024. J Clin Invest. :134. PubMed Chen Y, et al. 2022. Stem Cells Int. 2022:1309684. PubMed\u003cbr\u003e\nRRID: AB_2632657 (BioLegend Cat. No. 108315) AB_2632658 (BioLegend Cat. No. 108316)\u003cbr\u003e\nStructure: Aminopeptidase A, 140 kD\u003cbr\u003e\nDistribution: B progenitors, bone marrow stromal lines, thymic dendritic cells, cortical epithelial cells\u003cbr\u003e\nFunction: Aminopeptidase, may function in IL-7-driven proliferation of B cell precursors\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Epithelial cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nAntigen References: 1. Goverman J, et al. 1997. Immunol. Today 18:204. 2. Morse HC, et al. 1987. J. Exp. Med. 165:920. 3. Sherwood P, et al. 1990. Int. Immunol. 2:399. 4. Lin Q, et al. 1998. J. Immunol. 160:4681.\u003cbr\u003e\nGene ID: 13809\u003cbr\u003e\nUniProt: View information about Ly-51 on UniProt.org\u003cbr\u003e\nClone: 6C3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: 6C3\/BP-1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862474477737,"sku":"108315","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-108315","provider":"Iright","version":"1.0","type":"link"}