{"product_id":"biolegend-108906","title":"Biolegend, 108906, FITC anti-mouse CD49b (pan-NK cells) Antibody, 500μg","description":"\u003cp\u003eDX5 antigen has been recently characterized as CD49b. It is a 150 kD integrin α chain also known as α 2 integrin, VLA-2 α chain, and integrin α 2 chain. CD49b non-covalently associates with CD29 (β 1 integrin) to form the CD49b\/CD29 complex known as VLA-2, a receptor for collagen and laminin. CD49b is expressed on platelets, the majority of NK cells, NKT cells, and a small subset of CD8+ T cells (this population can be significantly increased following viral infection). DX5 is used for the identification and isolation of NK cells, and is especially useful for identifying NK cells in mice lacking the NK1.1 antigen.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: IL-2-propagated NK1.1+ cells from C57BL\/6 mice\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: The DX5 clone detects cells expressing relatively high levels of CD49b and may not be useful for the detection of cells expressing low levels of CD49b. DX5 does not block NK cell killing or binding to collagen in vitro. Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity2 and immunohistochemical staining5 of formalin-fixed and paraffin-embedded tissue sections as well as immunohistochemical staining of acetone-fixed frozen sections10. The binding of DX5 antibody to splenic NK cells can be blocked by HMa2 antibody.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Arase H, et al. 2001. J. Immunol. 167:1141. (FC) Sepulveda H, et al. 1999. J. Immunol. 163:1133. Norian LA and Allen PM. 2004. J. Immunol. 173:835. (FC) Andoniou CE, et al. 2005. Nature Immunology 6:1011. Oertelt S, et al. 2006. J. Immunol. 177:1655. (IHC) PubMed Bourdeau A, et al. 2007. Blood doi:10.1182\/blood-2006-08-044370. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed Busche A, et al. 2011. J. Immunol. 186:2918. PubMed Kim HR, et al. 2011. Nephrology 16:545. (IHC) PubMed Seyoum B, et al. 2011. Vaccine. 29:8002. PubMed Younos IH, et al. 2012. Int Immunopharmacol. 13:245. PubMed Honjo K, et al. 2012. PNAS. PubMed. Huang HN, et al. 2013. Biomaterials. 34:10151. PubMed\u003cbr\u003e\nProduct Citations: Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed Pi C, et al. 2022. Cancers (Basel). 14:. PubMed Briukhovetska D, et al. 2023. Immunity. 56:143. PubMed Kurelac I, et al. 2019. Nat Commun. 10:903. PubMed Johnson KD, et al. 2022. Blood Adv. 6:1464. PubMed Wu L, et al. 2022. Front Immunol. 13:860761. PubMed Sharma NS, et al. 2020. J Clin Invest. 130:451. PubMed An SB, et al. 2022. Nat Commun. 13:5669. PubMed Chung YM, et al. 2022. Am J Cancer Res. 12:1241. PubMed Luff DH, et al. 2021. Front Immunol. 631271:12. PubMed Kuns R, et al. 2009. Blood. 113:5999. PubMed Köchl R, et al. 2020. Elife. 9:00. PubMed Niemann J, et al. 2019. Nat Commun. 10:3236. PubMed Alikhanyan K, et al. 2021. Cancers (Basel). 13:. PubMed Synn CB, et al. 2022. Clin Transl Immunology. 11:e1364. PubMed Meadows D, et al. 2015. PLoS One. 10: 0143738. PubMed Chung YM, et al. 2021. J Immunother Cancer. 9:. PubMed Weigmann B, et al. 2012. J Allergy Clin Immunol. 129:1126. PubMed Liu Y, et al. 2017. Oncogene. 10.1038\/onc.2017.209. PubMed Zhang W, et al. 2020. Nat Commun. 11:1187. PubMed Ryan NM, et al. 2022. Front Immunol. 13:932742. PubMed Hirata SI, et al. 2020. Allergy. 75:1939. PubMed Kim DK, et al. 2022. Nat Commun. 13:6292. PubMed Younes AI, et al. 2021. Transl Oncol. 14:100983. PubMed Soria-Castro R, et al. 2020. Sci Rep. 10:17802. PubMed Valkenburg S, et al. 2016. Sci Rep. 6:22666. PubMed Silva DA, et al. 2019. Nature. 565:186. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Yoshida H, et al. 2019. Cell. 176:897. PubMed Zhang Z, et al. 2020. Nature. 579:415. PubMed Fedele C, et al. 2021. J Exp Med. 218: . PubMed Kumar D, et al. 2018. Cancer Prev Res (Phila). 0.895138889. PubMed Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed Agelidis A, et al. 2017. Cell Rep. 10.1016\/j.celrep.2017.06.041. PubMed Mazumder S, et al. 2021. Heliyon. 7:e08124. PubMed Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed\u003cbr\u003e\nRRID: AB_313412 (BioLegend Cat. No. 108905) AB_313413 (BioLegend Cat. No. 108906)\u003cbr\u003e\nStructure: Integrin α chain, 150 kD\u003cbr\u003e\nDistribution: NK cells, subset of T cells\u003cbr\u003e\nFunction: Adhesion\u003cbr\u003e\nLigand\/Receptor: Collagen, laminin\u003cbr\u003e\nCell Type: NK cells, T cells\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Arase H, et al. 2001. J. Immunol. 167:1141. 2. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 3. Sasaki K, et al. 2003. Int. Immunol. 15:701. 4. Inoue O, et al. 2003. J. Cell Biol. 160:769.\u003cbr\u003e\nGene ID: 16398\u003cbr\u003e\nUniProt: View information about CD49b on UniProt.org\u003cbr\u003e\nClone: DX5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: α2 integrin, VLA-2 α chain, DX5, Integrin α2 chain, ITGA2\u003cbr\u003e\nIsotype: Rat IgM, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863062007977,"sku":"108906","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-108906","provider":"Iright","version":"1.0","type":"link"}