{"product_id":"biolegend-109804","title":"Biolegend, 109804, Biotin anti-mouse CD45.2 Antibody, 500μg","description":"\u003cp\u003eCD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB\/c, CBA\/Ca, CBA\/J, C3H\/He, C57BL, C57BR, C57L, C58, DBA\/1, DBA\/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: B10.S mouse thymocytes and splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Suzuki K, et al. 2000. Immunity 13:691. (IHC) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837. Pascal V, et al. 2007. J. Immunol. 179:1751. Burman AC, et al. 2007. Blood 110:1064. Kincaid EZ, et al. 2007. J. Immunol. 179:3187. Phan TG, et al. 2007. Nature Immunol. 8:992. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed Wen T, et al. 2013. PNAS. 110:6067. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed\u003cbr\u003e\nProduct Citations: Pisterzi P, et al. 2023. Hemasphere. 7:e823. PubMed Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed McLelland B, et al. 2011. J Immunol Methods. 367:85. PubMed Bromley SK, et al. 2020. Cell Reports. 32(9):108085. PubMed Yang K, et al. 2018. J Virol. 92:e00428. PubMed Fidler TP, et al. 2021. Nature. 592:296. PubMed Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed Zhao L, et al. 2020. EBioMedicine. 60:103020. PubMed Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed Wolock SL, et al. 2019. Cell Rep. 28:302. PubMed Mesa-Nuñez C, et al. 2022. Mol Ther Methods Clin Dev. 26:459. PubMed Kopinke D et al. 2017. Cell. 170(2):340-351 . PubMed Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed\u003cbr\u003e\nRRID: AB_313440 (BioLegend Cat. No. 109803) AB_313441 (BioLegend Cat. No. 109804)\u003cbr\u003e\nStructure: Protein tyrosine phosphatase (PTP) family, 180-240 kD\u003cbr\u003e\nDistribution: All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice\u003cbr\u003e\nFunction: Phosphatase, T and B cell activation\u003cbr\u003e\nLigand\/Receptor: Galectin-1, CD2, CD3, CD4\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Suzuki K, et al. 2000. Immunity 13:691.\u003cbr\u003e\nGene ID: 19264\u003cbr\u003e\nUniProt: View information about CD45.2 on UniProt.org\u003cbr\u003e\nClone: 104\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Ly-5.2, LCA\u003cbr\u003e\nIsotype: Mouse (SJL) IgG2a, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860770771113,"sku":"109804","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-109804","provider":"Iright","version":"1.0","type":"link"}