{"product_id":"biolegend-109820","title":"Biolegend, 109820, Pacific Blue™ anti-mouse CD45.2 Antibody, 100μg","description":"\u003cp\u003eCD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB\/c, CBA\/Ca, CBA\/J, C3H\/He, C57BL, C57BR, C57L, C58, DBA\/1, DBA\/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: B10.S mouse thymocytes and splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Suzuki K, et al. 2000. Immunity 13:691. (IHC) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837. Pascal V, et al. 2007. J. Immunol. 179:1751. Burman AC, et al. 2007. Blood 110:1064. Kincaid EZ, et al. 2007. J. Immunol. 179:3187. Phan TG, et al. 2007. Nature Immunol. 8:992. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed Wen T, et al. 2013. PNAS. 110:6067. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed\u003cbr\u003e\nProduct Citations: Valladao A, et al. 2016. J Immunol. 197(12):4541-4551. 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Eur J Immunol. 51:1968. PubMed Lin G, et al. 2013. PLoS One. 8:e56014. PubMed Zhu Y, et al. 2015. Neurobiol Dis. 74:114. PubMed Mizraji* G, et al. 2013. J Vis Exp. 77: 50388. PubMed Tuttle KD, et al. 2020. Cell Rep. 33:108407. PubMed Koren N, et al. 2021. Cell Host Microbe. 29(2):197-209.e5. PubMed Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed Flommersfeld S, et al. 2021. Immunity. :. PubMed Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed Goldman E, et al. 2021. Cells. 10:. PubMed Dewas C, et al. 2015. J Immunol. 194:1372. PubMed Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed Chen X, et al. 2019. Nat Commun. 4.421527778. PubMed Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed Kincaid E, et al. 2007. J Immunol. 179:3187. PubMed Patel S, et al. 2019. Viruses. 0.877083333. 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Proc Natl Acad Sci U S A. 111:3799. PubMed Knier B, et al. 2018. Nat Immunol. 1.722916667. PubMed\u003cbr\u003e\nRRID: AB_492873 (BioLegend Cat. No. 109819) AB_492872 (BioLegend Cat. No. 109820)\u003cbr\u003e\nStructure: Protein tyrosine phosphatase (PTP) family, 180-240 kD\u003cbr\u003e\nDistribution: All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice\u003cbr\u003e\nFunction: Phosphatase, T and B cell activation\u003cbr\u003e\nLigand\/Receptor: Galectin-1, CD2, CD3, CD4\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Suzuki K, et al. 2000. Immunity 13:691.\u003cbr\u003e\nGene ID: 19264\u003cbr\u003e\nUniProt: View information about CD45.2 on UniProt.org\u003cbr\u003e\nClone: 104\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Ly-5.2, LCA\u003cbr\u003e\nIsotype: Mouse (SJL) IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860771459241,"sku":"109820","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-109820","provider":"Iright","version":"1.0","type":"link"}