{"product_id":"biolegend-109827","title":"Biolegend, 109827, PerCP\/Cyanine5.5 anti-mouse CD45.2 Antibody, 25μg","description":"\u003cp\u003eCD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB\/c, CBA\/Ca, CBA\/J, C3H\/He, C57BL, C57BR, C57L, C58, DBA\/1, DBA\/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: B10.S mouse thymocytes and splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Suzuki K, et al. 2000. Immunity 13:691. (IHC) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837. Pascal V, et al. 2007. J. Immunol. 179:1751. Burman AC, et al. 2007. Blood 110:1064. Kincaid EZ, et al. 2007. J. Immunol. 179:3187. Phan TG, et al. 2007. Nature Immunol. 8:992. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed Wen T, et al. 2013. PNAS. 110:6067. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed\u003cbr\u003e\nProduct Citations: Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed Charpentier JC, et al. 2020. Nat Commun. 11:180. PubMed Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed Lee KM, et al. 2022. Cancer Immunol Res. 10:829. PubMed Li T, et al. 2022. J Leukoc Biol. 112:845. PubMed Song S, et al. 2023. Bioact Mater. 28:358. PubMed Wang Y, et al. 2021. Cancer Res. 81:174. PubMed Nozaki K, et al. 2022. Nature. 606:960. PubMed VanDyke D, et al. 2022. Cell Rep. 41:111478. PubMed Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed Vijver SV, et al. 2023. Front Immunol. 13:1100730. PubMed Sun Y, et al. 2023. Cell Mol Life Sci. 80:63. PubMed Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed Li X, et al. 2022. Cell. 185:1709. PubMed Emal D, et al. 2016. J Am Soc Nephrol. 10.1681\/ASN.2016030255. PubMed Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed Zhang LJ, et al. 2019. Immunity. 50:121. PubMed Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed Scalzo S, et al. 2022. Int J Nanomedicine. 17:2865. PubMed Low MSY, et al. 2019. Cell Reports. 29(9):2634-2645.e5. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Rothchild AC, et al. 2020. Bio Protoc. 10:00. PubMed Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed Rodriguez-Ruiz M, et al. 2016. Cancer Res . 76: 5994 - 6005. PubMed Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed Kleinholz CL, et al. 2021. Sci Rep. 11:15071. PubMed Yang F, et al. 2020. Cancer Res. 80:3677. PubMed Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed Samarchith P Kurup et al. 2019. Cell host \u0026amp; microbe. 25(4):565-577 . PubMed Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed Tian Y, et al. 2016. J Immunol. 196: 2153 - 2166. PubMed Yap JY, et al. 2018. Immunology. 154:522. PubMed Zhang K, et al. 2014. PLoS Pathog. 10:1004385. PubMed Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed Xu S, et al. 2021. Immunity. . PubMed Stotesbury C, et al. 2020. J Immunol. 204:1582. PubMed Kan X, et al. 2015. Cardiovasc Res. 10.1093\/cvr\/cvv255. PubMed Fraccarollo D, et al. 2021. eLife. 0.416666666666667. PubMed Olson GS, et al. 2021. Cell Reports. 35(9):109195. PubMed Tian Y, et al. 2016. J Immunol. 197: 1308 - 1321. PubMed Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed Giampazolias E, et al. 2021. Cell. . PubMed Evgin L, et al. 2022. Sci Transl Med. 14:eabn2231. PubMed MacKinnon A, et al. 2011. Am J Respir Crit Care Med. 185:537. PubMed Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed Wang X, et al. 2016. J Immunol. 197(12):4750-4761. PubMed Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed Forero A, et al. 2020. Immunity. 51(3):451-464. PubMed Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed Tang W, et al. 2021. Front Cell Dev Biol. 9:728057. PubMed Xu Y, et al. 2021. iScience. 24:103445. PubMed Cao H, et al. 2020. Front Pharmacol. 11:578943. PubMed Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed Cabal-Hierro L, et al. 2020. Nat Commun. 11:1406. PubMed\u003cbr\u003e\nRRID: AB_893352 (BioLegend Cat. No. 109827) AB_893350 (BioLegend Cat. No. 109828)\u003cbr\u003e\nStructure: Protein tyrosine phosphatase (PTP) family, 180-240 kD\u003cbr\u003e\nDistribution: All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice\u003cbr\u003e\nFunction: Phosphatase, T and B cell activation\u003cbr\u003e\nLigand\/Receptor: Galectin-1, CD2, CD3, CD4\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Suzuki K, et al. 2000. Immunity 13:691.\u003cbr\u003e\nGene ID: 19264\u003cbr\u003e\nUniProt: View information about CD45.2 on UniProt.org\u003cbr\u003e\nClone: 104\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Ly-5.2, LCA\u003cbr\u003e\nIsotype: Mouse (SJL) IgG2a, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860771917993,"sku":"109827","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-109827","provider":"Iright","version":"1.0","type":"link"}