{"product_id":"biolegend-110607","title":"Biolegend, 110607, PE anti-mouse H-2D, 50μg","description":"\u003cp\u003eThe 34-2-12 antibody recognizes α 3 domain of H-2D d MHC class I alloantigen expressed on nucleated cells from mice of the H-2D d haplotype. H-2D d is involved in antigen presentation to T cells expressing CD3\/TCR and CD8 proteins. The 34-2-12 antibody cross-reacts with cells from the C3H.LG\/Ckc strain. Reactivity with other haplotypes ( e.g., b,f,k,p,q,r,s) has not been reported. Clone 34-2-12 recognizes the α3 domain of D d .\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: (C57BL\/6 x DBA\/2)F1 mouse splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation2,3, blocking of cell adhesion4,5, and immunohistochemical staining of acetone-fixed frozen sections6. This clone does not cross react with other haplotypes (e.g., b, f, k, p, q, r, s).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ozato K, et al. 1982. Transplantation 34:113. McCluskey J, et al. 1985. Cell 40:247. McCluskey J, et al. 1986. J. Immunol. 136:1472. Kane KP. 1994. J. Exp. Med. 179:1011. Daniels BF, et al. 1994. J. Exp. Med. 180:687. Nikolic B, et al. 1999. J. Immunol. 162:3402. Ma XT, et al. 2006. Cancer Research 66:1169. Matsumoto N, et al. 1998. Immunity 8:245.\u003cbr\u003e\nProduct Citations: Jayachandran R, et al. 2019. Immunity. 50:152. PubMed Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed Parikh BA, et al. 2020. Cell Rep. 32:107969. PubMed Markey K, et al. 2014. J Immunol. 192:5426. PubMed Sivori S, et al. 2020. Cell Reports. 16(5):430-441. PubMed Cui Y, et al. 2014. J Immunol. 193:4783. PubMed Renner K, et al. 2020. Cell Reports. 29(1):135-150.e9.. PubMed Greeneltch K, et al. 2007. Cancer Res. 67:10406. PubMed Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed Zheng Y, et al. 2022. Transl Res. :. PubMed\u003cbr\u003e\nRRID: AB_313488 (BioLegend Cat. No. 110607) AB_313489 (BioLegend Cat. No. 110608)\u003cbr\u003e\nStructure: MHC class I\u003cbr\u003e\nDistribution: Nucleated cells of the H-2Dd haplotype\u003cbr\u003e\nFunction: Antigen presentation\u003cbr\u003e\nLigand\/Receptor: CD3\/TCR, CD8\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. 3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.\u003cbr\u003e\nGene ID: 14964\u003cbr\u003e\nUniProt: View information about H-2Dd on UniProt.org\u003cbr\u003e\nClone: 34-2-12\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MHC class I\u003cbr\u003e\nIsotype: Mouse (C3H\/HeJ) IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862375387305,"sku":"110607","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-110607","provider":"Iright","version":"1.0","type":"link"}