{"product_id":"biolegend-112803","title":"Biolegend, 112803, Biotin anti-mouse CD119 (IFN-γ R α chain) Antibody, 50μg","description":"\u003cp\u003eCDw119 is a 90 kD immunoglobulin superfamily member, also known as IFN-γRα chain. It is a class II cytokine receptor family member that serves as a IFN-γ-binding chain associated with the IFN-γ β chain also known as AF-1. In addition to ligand binding, CDw119 participates in ligand trafficking. CDw119 is expressed on T and B cells, NK cells, fibroblasts, endothelial, and epithelial cells. Binding of IFN-γ induces receptor dimerization, internalization, Jak1 and Jak2 protein kinase activation and, ultimately, STAT1 activation. IFN-γ initiates and regulates a variety of immune responses.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: Soluble recombinant mouse IFN-γR α chain protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: Western blotting and immunoprecipitation1. The 2E2 antibody does not block the binding of IFN-γ to IFN-γ receptor. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112804) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Bach EA, et al. 1995. Science 270:1215.\u003cbr\u003e\nProduct Citations: Tang Q, et al. 2022. Mol Ther. 30:2709. PubMed Kiritsy MC, et al. 2021. Elife. 10:. PubMed Bruin A, et al. 2010. Blood. 116:2559. PubMed Cella M, et al. 2010. Proc Natl Acad Sci U S A. 107:10961. PubMed Ji L et al. 2019. Immunity. 51(2):272-284 . PubMed DanDan Huang, Lang Bao 2016. J Microbiol Immunol Infect. 49:859-865. PubMed Ribechini E, et al. 2017. Blood Adv. 0.699305556. PubMed\u003cbr\u003e\nRRID: AB_2123476 (BioLegend Cat. No. 112803) AB_2123475 (BioLegend Cat. No. 112804)\u003cbr\u003e\nStructure: Ig superfamily, class II cytokine receptor family, associates with IFN-γ AF-1, 90 kD\u003cbr\u003e\nDistribution: T cells and B cells, NK cells, myeloid cells, fibroblasts, endothelial and epithelial cells\u003cbr\u003e\nFunction: Initiates and regulates immune response\u003cbr\u003e\nLigand\/Receptor: IFN-γ\u003cbr\u003e\nCell Type: B cells, Endothelial cells, Epithelial cells, Fibroblasts, NK cells, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Basu M, et al. 1988. P. Natl. Acad. Sci. USA 85:6282. 2. Codias EK, et al. 1990. J. Immunol. 144:2197.\u003cbr\u003e\nGene ID: 15979\u003cbr\u003e\nUniProt: View information about CD119 on UniProt.org\u003cbr\u003e\nClone: 2E2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IFN-γRα , IFN-γ Receptor α chain, CDw119\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862319714473,"sku":"112803","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-112803","provider":"Iright","version":"1.0","type":"link"}