{"product_id":"biolegend-115520","title":"Biolegend, 115520, PE\/Cyanine7 anti-mouse CD19 Antibody, 100μg","description":"\u003cp\u003eCD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse CD19-expressing K562 human erythroleukemia cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunofluorescence7.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Shoham T, et al. 2003. J. Immunol. 171:4062. (FC) Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC) Kamimura D, et al. 2006. J. Immunol. 177:306. (FC) Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC) Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC) Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC) Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed Olive V, et al. 2013. Elife. 2:822. PubMed Miyai T, et al. 2014. PNAS. 111:11780. PubMed\u003cbr\u003e\nProduct Citations: Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed Barrett N, et al. 2016. Cell Rep. 16: 1039-1054. PubMed Bussières-Marmen S, et al. 2017. Cell Mol Immunol. 10.1038\/cmi.2016.72. PubMed Turecamo SE, et al. 2018. Matrix Biol. 67:01:00. PubMed Agrafiotis A, et al. 2023. iScience. 26:106055. PubMed Wang Y, et al. 2023. EBioMedicine. 90:104480. PubMed Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed van Os BW, et al. 2023. Eur Heart J Open. 3:oead013. PubMed Prior JT, et al. 2023. NPJ Vaccines. 8:80. PubMed Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed Zhu Y, et al. 2022. Nat Commun. 13:4282. PubMed Friesen M, et al. 2022. Brain Pathol. 32:e13032. PubMed Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed Millar FR, et al. 2022. Cell Rep. 41:111596. PubMed Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed Lin YH, et al. 2021. Front Immunol. 12:626418. PubMed d'Errico P, et al. 2021. Nat Neurosci. Online ahead of print. PubMed Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed Clark K, et al. 2015. J Autoimmun. Available online 21 August 2015. PubMed Castañeda-Zegarra S, et al. 2020. Aging (Albany NY). 12:00. PubMed Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed Morgado P, et al. 2014. Infect Immun . 82:4047. PubMed Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed Liao Y, et al. 2017. Virol J. 10.1186\/s12985-017-0801-x. PubMed Yang C, et al. 2020. J Control Release. 326:324. PubMed Lin CA, et al. 2021. Pharmaceutics. 13:. PubMed Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed Campana L, et al. 2018. J Immunol. 200:1169. PubMed Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed Alberti D, et al. 2021. Curr Protoc. 1:e311. PubMed Rogawski DS, et al. 2021. Nat Commun. 12:2792. PubMed Ma M, et al. 2021. Front Immunol. 12:718779. PubMed Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed Syed A, et al. 2015. Infect Immun . 83: 3428-3437. PubMed Uchil PD et al. 2018. Cell host \u0026amp; microbe. 25(1):87-100 . PubMed Schaffert A, et al. 2020. Front Immunol. 10:3024. PubMed Yoshida K, et al. 2022. Sci Rep. 12:17276. PubMed Calés C, et al. 2016. Mol Cell Biol. 36: 900 - 912. PubMed JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed Qi T, et al. 2020. Cell Rep. 33:108452. PubMed Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed Pereira JA, et al. 2020. Hum Mol Genet. 29:1253. PubMed Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed Bradley KC, et al. 2019. Cell Rep. 28:245. PubMed Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Noviski M, et al. 2018. Elife. 7:e35074. PubMed Zhang H, et al. 2019. Mol Cell. 76:110. PubMed Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed Madenspacher JH, et al. 2020. JCI Insight. 5:00. PubMed Pillonel V, et al. 2016. Sci Rep. 6:37772. PubMed Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed Challa TD, et al. 2020. Cell Reports. 30(10):3424-3433. PubMed Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed Wu S, et al. 2022. BMC Immunol. 23:17. PubMed Kang X, et al. 2022. J Immunol Res. 2022:8118577. PubMed Kim MY, et al. 2018. Cell. 173:1439. PubMed Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed Shiozawa S, et al. 2022. iScience. 25:103537. PubMed Ma K, et al. 2022. iScience. 25:104347. PubMed Zhang P, et al. 2022. Nat Commun. 13:1588. PubMed Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed Zhou X, et al. 2021. Cell Reports. 34(1):108601. PubMed Li M, et al. 2021. Cancers (Basel). 13:. PubMed Wieghofer P, et al. 2021. EMBO J. 40:e105123. PubMed Zhou J, et al. 2022. Int J Oncol. 61: . PubMed Lehrke MJ, et al. 2021. Elife. 10:. PubMed Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed Onai N, et al. 2020. Int J Mol Sci. :22. PubMed Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed Ackermann C, et al. 2019. Sci Rep. 9:10624. PubMed Chen C, et al. 2020. Cell Rep. 2136:30. PubMed Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed Vincent B, et al. 2010. J Immunol. 184:4196. PubMed Ghezraoui H, et al. 2018. Nature. 560:122. PubMed Willemsen J, et al. 2021. Cell Rep. 37:109977. PubMed Korin B, et al. 2020. Sleep. :43. PubMed Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed Zhang Y, et al. 2022. Front Pharmacol. 13:952775. PubMed Boulch M, et al. 2021. Sci Immunol. 6:. PubMed Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed Zhang B, et al. 2021. Front Neurol. 607370:12. PubMed Kleiman E, et al. 2016. Proc Natl Acad Sci U S A. 113: E3911 - E3920. PubMed Fusciello M, et al. 2019. Nat Commun. 4.407638889. PubMed Huang LH et al. 2018. Cell metabolism. 29(2):475-487 . PubMed Song W, et al. 2022. Immunity. 55:290. PubMed\u003cbr\u003e\nRRID: AB_313654 (BioLegend Cat. No. 115519) AB_313655 (BioLegend Cat. No. 115520)\u003cbr\u003e\nStructure: Ig superfamily, associates with CD21 and CD81, 95 kD\u003cbr\u003e\nDistribution: Pro-B cells to mature B cells (during development), follicular dendritic cells\u003cbr\u003e\nFunction: Modulates B cell activation and differentiation\u003cbr\u003e\nLigand\/Receptor: CD21, CD81, Leu-13\u003cbr\u003e\nCell Type: B cells, Dendritic cells\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341. 2. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 3. Krop I, et al. 1996. J. Immunol. 157:48. 4. Tedder TF, et al. 1994. Immunol. Today 15:437.\u003cbr\u003e\nGene ID: 12478\u003cbr\u003e\nUniProt: View information about CD19 on UniProt.org\u003cbr\u003e\nClone: 6D5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: B4\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862476705961,"sku":"115520","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-115520","provider":"Iright","version":"1.0","type":"link"}