{"product_id":"biolegend-115706","title":"Biolegend, 115706, PE anti-mouse CD314 (NKG2D) Antibody, 200μg","description":"\u003cp\u003eNKG2D is a lectin-like type II transmembrane protein also known as CD314. It is expressed on NK cells, a subset of CD8 + T cells, γ\/δ T cells and NK1.1 + T cells, as well as in vitro induced LAK cells. NKG2D serves as a stimulatory immunoreceptor to activate NK cells via the non-covalently associated DAP10 or DAP12 adaptor. Several molecules have been identified as the ligands for NKG2D, including minor histocompatibility molecule, H60, UL16-binding protein-like transcript 1 (Mult1, and a family of retinoic acid early transcript 1 (Rae1) in mice, MHC class-I chain-related protein A (MICA), MICB, and UL16-binding proteins (ULBPs) in humans. present in both mice and humans. NKG2D ligands trigger cytokine (IFN-γ, GM-CSF, TNF-α, MIP1β and others) and granzyme release from NK cells. The C7 antibody has been reported to block NK cell killing of targets expressing NKG2D ligands and induce redirected lysis.\u003cbr\u003e\n200μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: Soluble mouse NKG2D\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported (for the relevant formats) applications include: blocking1,2,4,5 and inducing redirected cell lysis1,2. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16\/CD32, clone 93 (Cat. No. 101301\/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115706) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 115704) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115713 \u0026amp; 115714).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Gilfillan S, et al. 2002. Nat. Immunol. 3:1150. (Block) Ho EL, et al. 2002. J. Immunol. 169:3667. (Block) Maeda M, et al. 2004. J. Immunol. 172:6115. (FC) Andoniou CE, et al. 2005. Nature Immunology 6:1011. (Block) O'Leary JG, et al. 2006. Nature Immunology 7:507. (Block) Tang X, et al. 2006. J. Immunol. 177:7645. (FC)\u003cbr\u003e\nProduct Citations: Song Y, et al. 2020. Front Immunol. 11:558143. PubMed Markov SD, et al. 2021. Mol Cancer Ther. 20:2457. PubMed Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed\u003cbr\u003e\nRRID: AB_2133292 (BioLegend Cat. No. 115705)\u003cbr\u003e\nStructure: C-type lectin\u003cbr\u003e\nDistribution: NK and NK-T cells\u003cbr\u003e\nFunction: NK cytolytic killing of target cells expressing NKG2D ligands, costimulation NK cells\u003cbr\u003e\nLigand\/Receptor: Minor histocompatibility molecule H60, retinoic acid early inducible 1 (RAE-1) family\u003cbr\u003e\nCell Type: NK cells, NKT cells\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Vance RE, et al. 1999. J. Exp. Med. 190:1801. 2. Vance RE, et al. 1998. J. Exp. Med. 188:1841. 3. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755. 4. Yokoyama WM. 1998. Curr. Opin. Immunol. 10:298. 5. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150. 6. Ho EL, et al. 2002. J. Immunol. 169:3667.\u003cbr\u003e\nGene ID: 27007\u003cbr\u003e\nUniProt: View information about CD314 on UniProt.org\u003cbr\u003e\nClone: C7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD314\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862951153833,"sku":"115706","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-115706","provider":"Iright","version":"1.0","type":"link"}