{"product_id":"biolegend-116145","title":"Biolegend, 116145, Brilliant Violet 785™ anti-mouse CD54 Antibody, 50μg","description":"\u003cp\u003eCD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes\/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a\/CD18) and Mac-1 (CD11b\/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1\/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse NS-1 cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780\/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and CD54 functions1,2,4, immunohistochemical staining3 of acetone-fixed frozen sections, immunoprecipitation4, and Western blotting (non-reducing). The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 116131-116136).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kumaska T, et al. 1996. J. Clin. Invest. 97:2362. (Block) Horley KJ, et al. 1989. EMBO J. 8:2889. (Block) Burns AR, et al. 1994. J. Immunol. 153:3189. (IHC) Takei F, et al. 1985. J. Immunol. 134:1403. (Block, IP) Sumagin R, et al. 2010. J. Immunol. 184:5242. PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Seidler DG, et al. 2011 J. Immunol. 187:6108. PubMed Chacko AM, et al. 2011. Curr Opin Colloid Interface Sci. 16:215. PUbMed El-Assaad F, et al. 2013. Infect Immun. 81:3984. PubMed Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed\u003cbr\u003e\nRRID: AB_3083104 (BioLegend Cat. No. 116145)\u003cbr\u003e\nStructure: Ig superfamily, 90 kD\u003cbr\u003e\nDistribution: Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes\/macrophages, granulocytes, dendritic cells\u003cbr\u003e\nFunction: Immune reaction, inflammation, adhesion\u003cbr\u003e\nLigand\/Receptor: CD11a\/CD18 (LFA-1) or CD11b\/CD18 (Mac-1) and CD11c\/CD18, CD43, hyaluronan, fibrinogen\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Springer TA. 1990. Nature 346:425. 3. Rothlein R, et al. 1986. J. Immunol. 137:1270.\u003cbr\u003e\nGene ID: 15894\u003cbr\u003e\nUniProt: View information about CD54 on UniProt.org\u003cbr\u003e\nClone: YN1\/1.7.4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: ICAM-1, Ly-47\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864107372713,"sku":"116145","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-116145","provider":"Iright","version":"1.0","type":"link"}