{"product_id":"biolegend-116406","title":"Biolegend, 116406, FITC anti-mouse I-A, 500μg","description":"\u003cp\u003eThe AF6-120.1 antibody reacts with the I-A b MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 b bearing mice, and are involved in antigen presentation to T cells expressing CD3\/TCR and CD4 proteins. The AF6-120.1 antibody cross-reacts with H-2 k and H-2 u haplotypes; this antibody does not cross-react with other haplotypes (d, f, q, r, s).\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: C57BL\/10J splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of frozen sections (acetone-fixed5; OCT-embedded, ethanol-fixed sections7), immunofluorescence microscopy3 (including acetone-fixed epidermal sheets6), immunoprecipitation7,8. Directly conjugated antibody was used for IF in (3) and (6) and for IHC in (5). Does not react with other haplotypes (e.g., d, f, q, r, s).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Wall KA, et al. 1983. J. Immunol. 131:1056. (FC) Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. (FC) Inaba K, et al. 1998. J. Exp. Med. 188:2163 (IF) Hamrah P, et al. 2002. Invest Opthalmol Vis. Sci. 43:639 (IF) Buono C, et al. 2003. Arterioscler. Thromb. Vasc. Biol. 23:454. (IHC) Wang Z, et al. 2004. J. Immunol. 172:5924. (IHC IF) Nakagawa TY, et al. 1999. Immunity 10:207. (IP) Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC IP) PubMed Schneppenheim J, et al. 2013. J Exp Med. 210:41. PubMed.\u003cbr\u003e\nProduct Citations: Burghardt S, et al. 2013. J Immunol. 191:5574. PubMed Kumpunya S, et al. 2023. Front Immunol. 13:1010764. PubMed Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed Clarke F,et al. 2017. PLoS One. 10.1371\/journal.pone.0186625. PubMed Huang S, et al. 2011. J Immunol. 186:931. PubMed Nguyen T, et al. 2017. Clin Transl Immunology. 10.1038\/cti.2017.4. PubMed Jia X, et al. 2021. Clin Transl Immunology. 10:e1336. PubMed Xu F, et al. 2022. Cell Death Discov. 8:142. PubMed Škrnjug I, et al. 2014. PLoS One. 9:110150. PubMed Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed Cheng Y, et al. 2020. PLoS Pathog. 16:e1008569. PubMed Nahrendorf W, et al. 2021. eLife. 10:00. PubMed Ueda N, et al. 2006. Int Immunol. 1.720138889. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Renner K, et al. 2020. Cell Reports. 29(1):135-150.e9.. PubMed Tanaka S, et al. 2021. Am J Transplant. 21:353. PubMed Nabekura T, et al. 2020. Immunity. 96:52. PubMed Wang Y, et al. 2022. Nat Cell Biol. 24:230. PubMed\u003cbr\u003e\nRRID: AB_313724 (BioLegend Cat. No. 116405) AB_313725 (BioLegend Cat. No. 116406)\u003cbr\u003e\nStructure: MHC class II\u003cbr\u003e\nDistribution: B cell and activated T cells, APCs of H-2b mice\u003cbr\u003e\nFunction: Antigen presentation\u003cbr\u003e\nLigand\/Receptor: CD3\/TCR, CD4\u003cbr\u003e\nCell Type: Antigen-presenting cells, B cells, T cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Watts C. 1997. Annu. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.\u003cbr\u003e\nGene ID: 14961\u003cbr\u003e\nUniProt: View information about I-Ab on UniProt.org\u003cbr\u003e\nClone: AF6-120.1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MHC class II\u003cbr\u003e\nIsotype: Mouse (BALB\/c) IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862533656745,"sku":"116406","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-116406","provider":"Iright","version":"1.0","type":"link"}