{"product_id":"biolegend-116518","title":"Biolegend, 116518, APC anti-mouse H-2K, 100μg","description":"\u003cp\u003eThe AF6-88.5 antibody reacts with H-2K b MHC class I alloantigen expressed on nucleated cells from mice of the H-2K b haplotype. H-2K b is involved in antigen presentation to T cells expressing CD3\/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: C57BL mouse splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunohistochemical staining of acetone-fixed frozen sections3. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections. Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Loken MR, et al. 1982. J. Immunol. Methods 50:R85. Wall KA, et al. 1983. J. Immunol. 131:1056. Andersson M, et al. 1998. J. Immunol. 161:6475. Shao H, et al. 2005. J. Immunol. 175:1851. Hui S, et al. 2005. J. Immunol. 175:1851. PubMed Zhou K, et al. 2010. Cytotherapy. 12:735. PubMed Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed\u003cbr\u003e\nProduct Citations: Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed Sethumadhavan S, et al. 2017. PLoS One. . 10.1371\/journal.pone.0187314. PubMed Regev O, et al. 2022. Front Immunol. 13:849701. PubMed Pierini S, et al. 2021. J Immunother Cancer. 9:. PubMed Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed Gruber T, et al. 2020. JCI Insight. 5:00. PubMed Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed Perry JSA, et al. 2018. Immunity. 48:923. PubMed Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed Wang X, et al. 2011. Blood. 118:6426. PubMed Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed Canton J, et al. 2021. Nat Immunol. 22:140. PubMed Gurusamy D, et al. 2020. Cancer Cell. 37(6):818-833.e9. PubMed Oladiran O, et al. 2021. J Neuroinflammation. 77:18. PubMed Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed Kalbasi A, et al. 2020. Sci Transl Med. :12. PubMed Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed Chen X, et al. 2021. Cell Rep. 37:109991. PubMed Fujimoto K, et al. 2022. Sci Rep. 12:18313. PubMed Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed Yoshida S, et al. 2022. PLoS One. 17:e0264317. PubMed Freed-Pastor WA, et al. 2021. Cancer Cell. 39:1342. PubMed Gejman RS et al. 2018. eLife. 7 pii: e41090. PubMed\u003cbr\u003e\nRRID: AB_10568693 (BioLegend Cat. No. 116517) AB_10564404 (BioLegend Cat. No. 116518)\u003cbr\u003e\nStructure: MHC class I\u003cbr\u003e\nDistribution: Nucleated cells of the H-2Kb haplotype\u003cbr\u003e\nFunction: Antigen presentation\u003cbr\u003e\nLigand\/Receptor: CD3\/TCR, CD8\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Watts C. 1997. Ann. Review Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323. 3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.\u003cbr\u003e\nGene ID: 14972\u003cbr\u003e\nUniProt: View information about H-2Kb on UniProt.org\u003cbr\u003e\nClone: AF6-88.5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MHC class I\u003cbr\u003e\nIsotype: Mouse (BALB\/c) IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862534803625,"sku":"116518","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-116518","provider":"Iright","version":"1.0","type":"link"}