{"product_id":"biolegend-117423","title":"Biolegend, 117423, PerCP\/Cyanine5.5 anti-mouse CD278 (ICOS) Antibody, 25μg","description":"\u003cp\u003eThe 7E.17G9 antibody reacts with the 47-57 kD ICOS protein, also known as inducible costimulatory molecule, and H4. This protein is homologous to the CD28\/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes and can costimulate T cells and induce proliferation. In addition ICOS has been shown to be involved in humoral immune responses (B cell germinal center formation). The ICOS ligand, B7h\/B7RP-1 and B7-H2 is constitutively expressed in B cell areas of secondary lymphoid organs and can be induced in other tissues by LPS. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development. ICOS stimulation has been shown to be involved in airway tolerance and the downregulation of pulmonary inflammation.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse ICOS cDNA and ICOS hexahistidine fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: blocking of ligand binding. The LEAF™ format is suggested for blocking studies.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Akbari O, et al. 2002. Nat. Med. 8:1024. Harada H, et al. 2003. J. Clin. Invest. 112:234. McAdam AJ, et al. 2000. J. Immunol. 165:5035. (FC Block) Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed\u003cbr\u003e\nRRID: AB_2832418 (BioLegend Cat. No. 117423) AB_2832419 (BioLegend Cat. No. 117424)\u003cbr\u003e\nStructure: CD28\/CTLA-4, 47-57kD\u003cbr\u003e\nDistribution: Activated T cells, subset of thymocytes\u003cbr\u003e\nFunction: Costimulates T cell activation, proliferation, humoral immune response\u003cbr\u003e\nLigand\/Receptor: B7h\/B7RP-1\/GL-50\u003cbr\u003e\nCell Type: T cells, Thymocytes\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Rudd CE, et al. 2003. Nat. Rev. Immunol. 3:544. 2. McAdam AJ, et al. 2000. J. Immunol. 165:5035. 3. Mak TW, et al. 2003. Nat. Immunol. 4:765\u003cbr\u003e\nGene ID: 54167\u003cbr\u003e\nUniProt: View information about CD278 on UniProt.org\u003cbr\u003e\nClone: 7E.17G9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Inducible COStimulatory molecule, H4\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862487748777,"sku":"117423","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-117423","provider":"Iright","version":"1.0","type":"link"}