{"product_id":"biolegend-121221","title":"Biolegend, 121221, PerCP anti-mouse CD43 Activation-Associated Glycoform Antibody, 25μg","description":"\u003cp\u003eCD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. Due to variable glycosylation and sialylation, two isoforms of CD43 have been identified. The 115 kD glycoform of CD43 is expressed on most hematopietic cells including T lymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5 + B cells. It is not present on resting B cells and erythrocytes. While the 130 kD glycoform is thought to be activation-associated form primarily expressed on myeloid cells, pre-B cells, and activated T cells. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors. The 1B11 antibody reacts with the activation-associated glycoform of CD43. The epitope recognized by 1B11 is also shared with desialylated CD45RB. This antibody is useful for differentiation of effector CD8 T cells and memory T cells.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse WEHI 274.3 myeloid tumor cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, and immunohistochemistry of acetone-fixed frozen sections.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Jones AT, et al. 1994. J. Immunol. 153:3426. (IP, WB) Carlow DA, et al. 1999. J. Immunol. 163:1441. (IP, WB) Onami TM, et al. 2002. J. Immunol. 168:6022. van der Most RG, et al. 2003. Intl. Immunol. 15:119. Chu VT, et al. 2007. J. Immunol. 179:5947. Lang A, et al. 2008. J. Immunol. 180:4848. PubMed Gibbert K, et al. 2010. J. Immunol. 185:6179. PubMed\u003cbr\u003e\nProduct Citations: Akhmetzyanova I, et al. 2016. J Immunol. 196: 484 - 492. PubMed Miguel R, et al. 2012. J Immunol. 189:3449. PubMed Klein JC, et al. 2017. Nat Commun. 8:14600. PubMed Lehrke MJ, et al. 2021. Elife. 10:. PubMed\u003cbr\u003e\nRRID: AB_893333 (BioLegend Cat. No. 121222)\u003cbr\u003e\nStructure: Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 115 kD and 130 kD glycoforms with different glycosylation and sialylation\u003cbr\u003e\nDistribution: Most hematopoietic cells including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, CD5+ B-1 cells and activated B cells, and hemotopoietic progenitor cells.\u003cbr\u003e\nFunction: Play a dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, induce apoptosis of T cells and hematopoietic progenitors\u003cbr\u003e\nLigand\/Receptor: CD54, Siglec-1\u003cbr\u003e\nCell Type: B cells, Granulocytes, Hematopoietic stem and progenitors, Monocytes, NK cells, Plasma cells, Platelets, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. van den Berg TK, et al. 2001. J. Immunol. 166:3637. 2. Moore T, et al. 1994. J. Immunol. 153:4978. 3. Onami TM, et al. 2002. J. Immunol. 168:6022. 4. Tong J, et al. 2004. J. Exp. Med. 199:1277. 5. Jones AT, et al. 1994. J. Immunol. 153:3426.\u003cbr\u003e\nGene ID: 20737\u003cbr\u003e\nUniProt: View information about CD43 on UniProt.org\u003cbr\u003e\nClone: 1B11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, gp115\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860844662953,"sku":"121221","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-121221","provider":"Iright","version":"1.0","type":"link"}