{"product_id":"biolegend-121402","title":"Biolegend, 121402, Purified anti-mouse CD103 Antibody, 200μg","description":"\u003cp\u003eCD103 is a type I transmembrane glycoprotein known as αE integrin or Integrin α IEL chain. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. T regulatory cells express high level of CD103. The CD103 expression on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β 7 , CD103 is expressed as αE\/β 7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.\u003cbr\u003e\n200μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: Mouse intestinal intraepithelial lymphocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality testedIHC-F, IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining1,7 of acetone-fixed frozen sections, immunofluorescence2, and in vitro activation1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): LeFrancois L, et. al, 1994. Eur. J. Immunol. 24:635. (FC, IHC, IP) Mysorekar IU, et. al, 2002. J. Biol. Chem. 277:37811. (FC, IF) Mikami N, et al. 2011. J. Immunol. 186:6886. PubMed del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed Xiao B, et al. 2015. PLoS One 1:e0115333. (IHC)\u003cbr\u003e\nProduct Citations: Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Blériot C, et al. 2020. Methods Mol Biol. 2164:87. PubMed Yu H, et al. 2020. Front Immunol. 11:1773. PubMed Li H, et al. 2022. Cancer Cell. 40:36. PubMed Feriotti C, et al. 2022. Cell Rep. 40:111167. PubMed Bajaña S, et al. 2021. Front Immunol. 11:577718. PubMed Mikami N, et al. 2011. J Immunol. 186:6886. PubMed Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed Tian D, et al. 2020. FASEB J. 34:3367. PubMed Orecchioni M, et al. 2022. Methods Mol Biol. 2419:779. PubMed Jackson JW, et al. 2021. Mol Ther Oncolytics. 22:444. PubMed Li W, et al. 2020. J Clin Invest. 130:6718. PubMed Chudnovskiy A et al. 2016. Cell. 167(2):444-456 . PubMed Lai JF, et al. 2020. J Allergy Clin Immunol. 146:1406. PubMed Liu H, et al. 2020. J Immunol. 205:1207. PubMed Hoves S, et al. 2018. J Exp Med. 215:859. PubMed Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed Schuler CF, et al. 2020. Allergy. 75:2279. PubMed Blriot C, et al. 2021. Immunity. :. PubMed Yao Y, et al. 2017. Nat Commun. 8:15402. PubMed Janela B, et al. 2019. Immunity. 50:1069. PubMed Gubin MM, et al. 2018. Cell. 175:1014. PubMed Ford J, et al. 2019. Front Immunol. 2.502083333. PubMed Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed Vander B, et al. 2017. J Cell Biol. 10.1083\/jcb.201512012. PubMed McClellan BL, et al. 2022. STAR Protoc. 3:101357. PubMed Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed Maier B, et al. 2020. Nature. 580:257. PubMed Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed\u003cbr\u003e\nRRID: AB_535944 (BioLegend Cat. No. 121401) AB_535945 (BioLegend Cat. No. 121402)\u003cbr\u003e\nStructure: Type I transmembrane glycoprotein, Integrin family, can be cleaved into 150 kD and 25 kD chains, associated with β7 integrin\u003cbr\u003e\nDistribution: Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells.\u003cbr\u003e\nFunction: Retention and activation of CD103+ lymphocytes in the intestinal epithelium, regulate tissue-specific T cell homing.\u003cbr\u003e\nLigand\/Receptor: E-Cadherin\u003cbr\u003e\nCell Type: Dendritic cells, Lymphocytes, T cells, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Kilshaw PJ and SJ. Murant. 1990. Eur. J. Immunol. 20:2201. 2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852. 3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635. 4. Sung SS, et al. 2006. J. Immunol. 176:2161. 5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063. 6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.\u003cbr\u003e\nGene ID: 16407\u003cbr\u003e\nUniProt: View information about CD103 on UniProt.org\u003cbr\u003e\nClone: 2E7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Integrin αIEL chain, Integrin αE chain, αE integrin, ITGAE\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862321221801,"sku":"121402","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-121402","provider":"Iright","version":"1.0","type":"link"}