{"product_id":"biolegend-121416","title":"Biolegend, 121416, PerCP\/Cyanine5.5 anti-mouse CD103 Antibody, 100μg","description":"\u003cp\u003eCD103 is a type I transmembrane glycoprotein known as αE integrin or Integrin α IEL chain. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. T regulatory cells express high level of CD103. The CD103 expression on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β 7 , CD103 is expressed as αE\/β 7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: Mouse intestinal intraepithelial lymphocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining1,7 of acetone-fixed frozen sections, immunofluorescence2, and in vitro activation1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): LeFrancois L, et. al, 1994. Eur. J. Immunol. 24:635. (FC, IHC, IP) Mysorekar IU, et. al, 2002. J. Biol. Chem. 277:37811. (FC, IF) Mikami N, et al. 2011. J. Immunol. 186:6886. PubMed del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed Xiao B, et al. 2015. PLoS One 1:e0115333. (IHC)\u003cbr\u003e\nProduct Citations: Quinn K, et al. 2013. J Immunol. 191:5085. PubMed Ender F, et al. 2017. PLoS One. 12(2):e0172446. PubMed Sauter M, et al. 2022. STAR Protoc. 3:101664. PubMed Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Michalaki C, et al. 2022. Curr Protoc. 2:e505. PubMed Ritzel R, et al. 2016. J Immunol. 196: 3318-3330. PubMed Verhagen J, et al. 2013. PLoS One. 8:61334. PubMed Wang D, et al. 2018. Immunity. 48:659. PubMed Sauter M, et al. 2022. iScience. 25:103677. PubMed Wong E, et al. 2019. Cell Rep. 29:3047. PubMed Khandelwal P, et al. 2013. PLoS One. 8:64193. PubMed Wiese A, et al. 2017. PLoS One. 10.1371\/journal.pone.0184956. PubMed Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed Karsten C, et al. 2015. J Immunol. 194:1841. PubMed Blijswijk J, et al. 2015. J Immunol. 194:307. PubMed Zhang J, et al. 2022. Front Immunol. 13:931740. PubMed Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed Cuburu N, et al. 2019. J Immunol. 202:1250. PubMed Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed Pérez-Girón J, et al. 2015. J Vis Exp. 100: 52803. PubMed Boucard-Jourdin M, et al. 2016. J Immunol. 197: 1968 - 1978. PubMed Port JR, et al. 2020. J Virol. 94:. PubMed Becker W, et al. 2021. J Crohns Colitis. 15:1032. PubMed Huang S, et al. 2016. Immunol Cell Biol. 10.1038\/icb.2016.75. PubMed Giampazolias E, et al. 2021. Cell. . PubMed Hoffmann FM, et al. 2018. Front Immunol. 9:1046. PubMed Kelly SH, et al. 2021. ACS Biomater Sci Eng. 7:1876. PubMed Luck H, et al. 2019. Nat Commun. 10:3650. PubMed Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed Penke LR, et al. 2020. Life Sci Alliance. 3:. PubMed Yasuda T, et al. 2016. PLoS One. 11:e0167952. PubMed Da Mesquita S, et al. 2021. Science Advances. 7(21):. PubMed Meunier I, et al. 2015. PLoS One. 10: 0138055. PubMed Reed HO, et al. 2019. J Clin Invest. 130:2514. PubMed Pavelko K, et al. 2017. Front Immunol. . 10.3389\/fimmu.2017.01532. PubMed Gaylo‐Moynihan A et al. 2019. Immunity. 51(2):298-309 . PubMed Kautz-Neu K, et al. 2011. J Exp Med. 208:885. PubMed Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed Sheng J, et al. 2021. eLife. 10:00. PubMed Stotesbury C, et al. 2020. Aging Cell. 19:e13170. PubMed Lian M, et al. 2015. PLoS One. 10: 0132741. PubMed Waaler J, et al. 2020. Commun Biol. 3:196. PubMed Chen R, et al. 2021. Front Oncol. 11:743050. PubMed Spath S, et al. 2022. iScience. 25:104998. PubMed\u003cbr\u003e\nRRID: AB_1574957 (BioLegend Cat. No. 121415) AB_2128621 (BioLegend Cat. No. 121416)\u003cbr\u003e\nStructure: Type I transmembrane glycoprotein, Integrin family, can be cleaved into 150 kD and 25 kD chains, associated with β7 integrin\u003cbr\u003e\nDistribution: Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells.\u003cbr\u003e\nFunction: Retention and activation of CD103+ lymphocytes in the intestinal epithelium, regulate tissue-specific T cell homing.\u003cbr\u003e\nLigand\/Receptor: E-Cadherin\u003cbr\u003e\nCell Type: Dendritic cells, Lymphocytes, T cells, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Kilshaw PJ and SJ. Murant. 1990. Eur. J. Immunol. 20:2201. 2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852. 3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635. 4. Sung SS, et al. 2006. J. Immunol. 176:2161. 5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063. 6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.\u003cbr\u003e\nGene ID: 16407\u003cbr\u003e\nUniProt: View information about CD103 on UniProt.org\u003cbr\u003e\nClone: 2E7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Integrin αIEL chain, Integrin αE chain, αE integrin, ITGAE\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862321123497,"sku":"121416","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-121416","provider":"Iright","version":"1.0","type":"link"}