{"product_id":"biolegend-122009","title":"Biolegend, 122009, PE anti-mouse CD28 Antibody, 50μg","description":"\u003cp\u003eCD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The E18 antibody blocks the binding of 37.51 antibody to CD28 and costimulates T cell activation and proliferation.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: A20 cells and recombinant mCD28Ig\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity, and in vitro induction of thymocyte differentiation. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 122004).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Dennehy KM, et al. 2006. J. Immunol. 176:5725.\u003cbr\u003e\nProduct Citations: Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed Dervović D, et al. 2012. J Immunol. 189:3411. PubMed Fennell LM, et al. 2020. EMBO J. 39:e103303. PubMed\u003cbr\u003e\nRRID: AB_604078 (BioLegend Cat. No. 122010)\u003cbr\u003e\nStructure: Ig superfamily, 44 kD\u003cbr\u003e\nDistribution: Thymocytes, CD4+, CD8+ peripheral T cells, NK cells\u003cbr\u003e\nFunction: Costimulates T and NK cells\u003cbr\u003e\nLigand\/Receptor: CD80 (B7-1), CD86 (B7-2)\u003cbr\u003e\nCell Type: NK cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233. 3. Gross JA, et al. 1992. J. Immunol. 149:380.\u003cbr\u003e\nGene ID: 12487\u003cbr\u003e\nUniProt: View information about CD28 on UniProt.org\u003cbr\u003e\nClone: E18\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Tp44, T44\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863065514153,"sku":"122009","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-122009","provider":"Iright","version":"1.0","type":"link"}