{"product_id":"biolegend-122520","title":"Biolegend, 122520, Pacific Blue™ anti-mouse Ly-6A\/E (Sca-1) Antibody, 100μg","description":"\u003cp\u003eLy-6A\/E is an 18 kD member of the Ly-6 multigene family also known as Sca-1. Ly6A\/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA\/2, SJL, SWR, and 129), Ly-6A\/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB\/c, CBA, C3H\/He, DBA\/1, and NZB) have low Ly-6A\/E expression on resting peripheral lymphocytes. The expression of Ly-6A\/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A\/E is thought to be involved in the regulation of both T and B cell responses. The E13-161.7 antibody has been reported to be useful for immunohistochemical staining of frozen sections, immunoprecipitation, flow cytometry, and complement-mediated cytotoxicity.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: mouse pre-T cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The Ly-6A\/E antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1, immunohistochemical staining of frozen sections2 and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD) Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC) van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP) van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF) Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294. Artinger EL, et al. 2013. PNAS. 110:12000. PubMed\u003cbr\u003e\nProduct Citations: Hu W, et al. 2015. Blood. 125:2206. PubMed Barrett N, et al. 2016. Cell Rep. 16: 1039-1054. PubMed Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed Herrejon Chavez F, et al. 2023. Nat Commun. 14:2290. PubMed Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed Chen J, et al. 2022. STAR Protoc. 3:101849. PubMed Yuan O, et al. 2022. Sci Adv. 8:eabm9987. PubMed Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed Fossati V, et al. 2010. J Immunol. 184:1251. PubMed Karra L, et al. 2020. Oncogene. 6920:39. PubMed Wong J, et al. 2015. Elife. 3: 07839. PubMed Grassinger J, et al. 2009. Blood. 114:49:00. PubMed Liu Y, et al. 2021. Haematologica. Online ahead of print. PubMed Nguyen DTT, et al. 2020. Nat Commun. 11:2026. PubMed Eisele AS, et al. 2022. Elife. 11:. PubMed Sala D, et al. 2019. Nat Commun. 10:1796. PubMed Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed Courtney AH et al. 2017. Molecular cell. 67(3):498-511 . PubMed Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed Yankova E, et al. 2021. Nature. 593:597. PubMed Oben K, et al. 2017. PLoS One. 10.1371\/journal.pone.0169767. PubMed Cheng Y, et al. 2019. Cell Rep. 28:1703. PubMed Zhang H, et al. 2019. Mol Cell. 76:110. PubMed Tzelepis K, et al. 2018. Nat Commun. 9:5378. PubMed Gerdes P, et al. 2022. Nat Commun. 13:7470. PubMed Ortega-Molina A, et al. 2021. Cell Reports. 36(2):109372. PubMed Lee SG, et al. 2020. J Nucl Med. . PubMed Spevak CC, et al. 2020. Cell Stem Cell. 26(5):755-765. PubMed Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed Yue F, et al. 2022. Cell Rep. 38:110267. PubMed Park SM et al. 2018. Cell stem cell. 24(1):153-165 . PubMed Gozdecka M, et al. 2018. Nat Genet. 50:883. PubMed Ellis S, et al. 2011. Blood. 118:1516. PubMed Jee D et al. 2018. Molecular cell. 69(2):265-278 . PubMed\u003cbr\u003e\nRRID: AB_2250602 (BioLegend Cat. No. 122519) AB_2143237 (BioLegend Cat. No. 122520)\u003cbr\u003e\nStructure: Ly-6 multigene family, 18 kD\u003cbr\u003e\nDistribution: Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells\u003cbr\u003e\nFunction: Regulates B and T cell responses\u003cbr\u003e\nCell Type: B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells\u003cbr\u003e\nBiology Area: Immunology, Stem Cells\u003cbr\u003e\nAntigen References: 1. Rock KL, et al. 1989. Immunol. Rev. 111:195. 2. Morrison SJ, et al. 1994. Immunity 1:661. 3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. 4. Malek T, et al. 1986. J. Exp. Med. 164:709.\u003cbr\u003e\nGene ID: 110454\u003cbr\u003e\nUniProt: View information about Ly-6A\/E on UniProt.org\u003cbr\u003e\nClone: E13-161.7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Sca-1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866087739561,"sku":"122520","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-122520","provider":"Iright","version":"1.0","type":"link"}