{"product_id":"biolegend-123408","title":"Biolegend, 123408, FITC anti-mouse CD21\/CD35 (CR2\/CR1) Antibody, 500μg","description":"\u003cp\u003eCD21, also known as CR2 (complement receptor 2) and C3d receptor, binds C3d and iC3b. It is also a receptor of Epstein-Barr virus. CD35, also known as CR1, binds C3b, iC3b, C4b, and iC4b. CD21\/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages, and activated granulocytes. CD21\/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81 and is involved in signal transduction.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: CD35\/CFA\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Boackle S, et al. 2001 Immunity 15:775. de Andres B, et al. 2012. J. Immunol. 189:2300. PubMed Chiu YK, et al. 2014. J Immunol. 193:2207. PubMed Koening PA, et al. 2014. J Biol Chem. 289:34490. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed Xu J, et al. 2021. Nature. 595:278. PubMed Yang Y, et al. 2021. Nat Commun. 12:525. PubMed Uchil PD et al. 2018. Cell host \u0026amp; microbe. 25(1):87-100 . PubMed Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed Hamilton J, et al. 2015. J Immunol. 194:5022. PubMed Li H, et al. 2020. Nature. 584:274. PubMed Ballesteros I, et al. 2020. Cell. 183(5):1282-1297.e18. PubMed Maulloo CD, et al. 2021. Front Immunol. 12:714842. PubMed Xu M, et al. 2013. J Immunol. 190:5436. PubMed Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed Shiozawa S, et al. 2022. iScience. 25:103537. PubMed Chen RJ, et al. 2022. iScience. 25:105595. PubMed Lu X, et al. 2019. Cell Rep. 28:472. PubMed Hamilton J,et al. 2017. J Immunol. 10.4049\/jimmunol.1700888. PubMed Yoshida H, et al. 2019. Cell. 176:897. PubMed Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed Chauvet S, et al. 2016. J Am Soc Nephrol. 27: 1665 - 1677. PubMed\u003cbr\u003e\nRRID: AB_940403 (BioLegend Cat. No. 123407) AB_940405 (BioLegend Cat. No. 123408)\u003cbr\u003e\nStructure: 145 kD\/190 kD type I transmembrane glycoprotein\u003cbr\u003e\nDistribution: B cells, follicular dendritic cells\u003cbr\u003e\nFunction: Signal transduction\u003cbr\u003e\nLigand\/Receptor: Associated with CD19\/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.\u003cbr\u003e\nCell Type: B cells, Dendritic cells\u003cbr\u003e\nBiology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Kozono Y, et al. 1998. J. Immunol. 160:1562. 2. Shimizu I, et al. 2007. Blood 109:1773. 3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.\u003cbr\u003e\nGene ID: 1290212946\u003cbr\u003e\nUniProt: View information about CD21\/CD35 on UniProt.org\u003cbr\u003e\nClone: 7E9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CR2\/CR1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862488502441,"sku":"123408","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-123408","provider":"Iright","version":"1.0","type":"link"}