{"product_id":"biolegend-123411","title":"Biolegend, 123411, APC anti-mouse CD21\/CD35 (CR2\/CR1) Antibody, 25μg","description":"\u003cp\u003eCD21, also known as CR2 (complement receptor 2) and C3d receptor, binds C3d and iC3b. It is also a receptor of Epstein-Barr virus. CD35, also known as CR1, binds C3b, iC3b, C4b, and iC4b. CD21\/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages, and activated granulocytes. CD21\/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81 and is involved in signal transduction.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: CD35\/CFA\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Boackle S, et al. 2001 Immunity 15:775. de Andres B, et al. 2012. J. Immunol. 189:2300. PubMed Chiu YK, et al. 2014. J Immunol. 193:2207. PubMed Koening PA, et al. 2014. J Biol Chem. 289:34490. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Chei S, et al. 2020. Front Nutr. 7:583186. PubMed Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed Lütge M, et al. 2023. Nat Immunol. . PubMed Laffleur B, et al. 2022. Sci Immunol. 7:eabn2738. PubMed Kodali S, et al. 2022. J Immunol. 208:1085. PubMed Wu J, et al. 2022. Nat Commun. 13:7321. PubMed Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed Petursdottir D, et al. 2017. Front Immunol. . 10.3389\/fimmu.2017.01699. PubMed Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed Parthasarathy R, et al. 2021. Front Immunol. 12:758407. PubMed Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed Rodda L, et al. 2015. J Immunol. 195: 4781 - 4791. PubMed Fennell LM, et al. 2020. EMBO J. 39:e103303. PubMed Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed Chiu Y, et al. 2014. J Immunol. 193:2207. PubMed Yunk L, et al. 2009. J Immunol. 183:452. PubMed Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed Koenig P, et al. 2014. J Biol Chem. 289:34490. PubMed Lehrke MJ, et al. 2021. Elife. 10:. PubMed Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed Andrés B, et al. 2012. J Immunol. 189:2300. PubMed Zane N, et al. 2012. J Immunol. 188:3009. PubMed Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed Silva N, et al. 2016. J Immunol. 196: 2591 - 2601. PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed\u003cbr\u003e\nRRID: AB_940395 (BioLegend Cat. No. 123411) AB_2085160 (BioLegend Cat. No. 123412)\u003cbr\u003e\nStructure: 145 kD\/190 kD type I transmembrane glycoprotein\u003cbr\u003e\nDistribution: B cells, follicular dendritic cells\u003cbr\u003e\nFunction: Signal transduction\u003cbr\u003e\nLigand\/Receptor: Associated with CD19\/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.\u003cbr\u003e\nCell Type: B cells, Dendritic cells\u003cbr\u003e\nBiology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Kozono Y, et al. 1998. J. Immunol. 160:1562. 2. Shimizu I, et al. 2007. Blood 109:1773. 3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.\u003cbr\u003e\nGene ID: 1290212946\u003cbr\u003e\nUniProt: View information about CD21\/CD35 on UniProt.org\u003cbr\u003e\nClone: 7E9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CR2\/CR1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862488600745,"sku":"123411","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-123411","provider":"Iright","version":"1.0","type":"link"}