{"product_id":"biolegend-123418","title":"Biolegend, 123418, APC\/Cyanine7 anti-mouse CD21\/CD35 (CR2\/CR1) Antibody, 100μg","description":"\u003cp\u003eCD21, also known as CR2 (complement receptor 2) and C3d receptor, binds C3d and iC3b. It is also a receptor of Epstein-Barr virus. CD35, also known as CR1, binds C3b, iC3b, C4b, and iC4b. CD21\/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages, and activated granulocytes. CD21\/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81 and is involved in signal transduction.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: CD35\/CFA\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Boackle S, et al. 2001 Immunity 15:775. de Andres B, et al. 2012. J. Immunol. 189:2300. PubMed Chiu YK, et al. 2014. J Immunol. 193:2207. PubMed Koening PA, et al. 2014. J Biol Chem. 289:34490. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Tan J, et al. 2021. iScience. 24(8):102835. PubMed Borbet TC, et al. 2023. iScience. 26:106810. PubMed Worth AN, et al. 2022. Cell Rep. 39:110899. PubMed Blanco LP, et al. 2022. Arthritis Rheumatol. 74:1971. PubMed Slobodin B, et al. 2020. Molecular Cell. 78(3):434-444.e5.. PubMed Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed Prados A, et al. 2021. Nat Immunol. 22:510. PubMed Riedel R, et al. 2020. Nat Commun. 11:2570. PubMed Kwok T, et al. 2022. Front Aging. 3:838943. PubMed Yin S et al. 2019. Cancer cell. 35(2):283-296 . PubMed Staffas A et al. 2018. Cell host \u0026amp; microbe. 23(4):447-457 . PubMed Prados A, Kollias G, Koliaraki V 2016. Sci Rep. 6: 33027. PubMed Smith LK, et al. 2021. Elife. 10:. PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed\u003cbr\u003e\nRRID: AB_1953274 (BioLegend Cat. No. 123417) AB_1953275 (BioLegend Cat. No. 123418)\u003cbr\u003e\nStructure: 145 kD\/190 kD type I transmembrane glycoprotein\u003cbr\u003e\nDistribution: B cells, follicular dendritic cells\u003cbr\u003e\nFunction: Signal transduction\u003cbr\u003e\nLigand\/Receptor: Associated with CD19\/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.\u003cbr\u003e\nCell Type: B cells, Dendritic cells\u003cbr\u003e\nBiology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Kozono Y, et al. 1998. J. Immunol. 160:1562. 2. Shimizu I, et al. 2007. Blood 109:1773. 3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.\u003cbr\u003e\nGene ID: 1290212946\u003cbr\u003e\nUniProt: View information about CD21\/CD35 on UniProt.org\u003cbr\u003e\nClone: 7E9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CR2\/CR1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862488699049,"sku":"123418","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-123418","provider":"Iright","version":"1.0","type":"link"}