{"product_id":"biolegend-124314","title":"Biolegend, 124314, PE\/Cyanine7 anti-mouse CD274 (B7-H1, PD-L1) Antibody, 100μg","description":"\u003cp\u003eCD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunofluorescence4, blocking6,7,8,9, and immunohistochemistry of acetone-fixed frozen sections4, 11. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 124303). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124318) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin \u0026lt;0.01 EU\/µg).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Maier H, et al. 2007. J. Immunol. 178:2714. Meng Q, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:4444. PubMed Scarlett UK, et al. 2012. J Exp Med. 209:495. PubMed Grabie N, et al. 2007. Circulation 116:2062. (IF, IHC) Paterson AM, et al. 2011. J. Immunol. 187:1097. Channappanavar R, et al. 2012. PLoS One 7:e39757. (Block) Schreiber HA, et al. 2010. PLoS One 5:e11453. (Block) PubMed Muthumani K, et al. 2011. J. Immunol. 187:2932. (Block) PubMed Cripps JG, et al. 2010. Hepatology 52:1350. (Block) PubMed Murakami R, et al. 2013. PLoS One. 8:73270. PubMed Riella LV, et al. 2011. Am. J. Transplant 11:832-40. (IHC) Lei GS, et al. 2015. Infect Immun. 83:572. PubMed\u003cbr\u003e\nProduct Citations: Zhao T, et al. 2023. J Immunother Cancer. 11:. PubMed Taha Z, et al. 2023. Front Immunol. 14:1181014. PubMed Li H, et al. 2022. Cancer Cell. 40:36. PubMed Yang H, et al. 2023. Cell Death Differ. 30:560. PubMed Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed Liu X, et al. 2020. Nature. . PubMed Harel M, et al. 2020. Cell. 179(1):236-250.e18.. PubMed Burrack AL, et al. 2019. Cell Rep. 28:2140. PubMed Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed Cai B, et al. 2021. Mol Cancer. 20:165. PubMed Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed Xu Y, et al. 2019. Nat Med. 25:301. PubMed Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed Wang GZ, et al. 2019. Nat Commun. 10:1125. PubMed Zhang D, et al. 2021. Cancer Med. 10:2137. PubMed Guo L, et al. 2021. Front Immunol. 12:785457. PubMed Li J, et al. 2018. Immunity. 49:178. PubMed Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed Zhao T, et al. 2022. JCI Insight. 7:. PubMed Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed Vogel A, et al. 2022. Cell Rep. 38:110420. PubMed Fang W, et al. 2021. J Exp Clin Cancer Res. 40:4. PubMed Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed Fedele C, et al. 2021. J Exp Med. 218: . PubMed Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed Kahn JH, et al. 2020. Endocrinology. 161(10):. PubMed Strait AA, et al. 2021. Commun Biol. 4:1005. PubMed Brenner E, et al. 2020. Nat Commun. 11:1335. PubMed Kaw S, et al. 2020. EMBO J. 39:e105594. PubMed\u003cbr\u003e\nRRID: AB_10639934 (BioLegend Cat. No. 124313) AB_10643573 (BioLegend Cat. No. 124314)\u003cbr\u003e\nStructure: 40 kD type I transmembrane protein member of B7 family within the immunoglobulin receptor superfamily\u003cbr\u003e\nDistribution: T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes\u003cbr\u003e\nLigand\/Receptor: PD-1 (PDCD1)\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Endothelial cells, Monocytes, NK cells, T cells\u003cbr\u003e\nBiology Area: Cancer Biomarkers, Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Sharpe A, et al. 2007. Nat. Immunol. 8:239. 2. Dong H, et al. 1999. Nat. Med. 5:1365. 3. Freeman G, et al. 2000. J. Exp. Med. 192:1027.\u003cbr\u003e\nGene ID: 60533\u003cbr\u003e\nUniProt: View information about CD274 on UniProt.org\u003cbr\u003e\nClone: 10F.9G2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: B7-H1, PD-L1\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860782239913,"sku":"124314","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-124314","provider":"Iright","version":"1.0","type":"link"}