{"product_id":"biolegend-125209","title":"Biolegend, 125209, APC anti-mouse CD223 (LAG-3) Antibody, 25μg","description":"\u003cp\u003eCD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Murine CD223-Ig fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA) Workman CJ, et al. 2005. J. Immunol. 174:688. (FC) Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)\u003cbr\u003e\nProduct Citations: Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed Zeng S, et al. 2023. Front Oncol. 13:1171926. PubMed Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed Ferrere G, et al. 2021. JCI Insight. 6:. PubMed Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed DeLong JH, et al. 2019. Immunohorizons. 3:13. PubMed Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed Sande O, et al. 2016. J Immunol. 196: 691 - 702. PubMed Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed Ma X et al. 2019. Cell Metab. 30(1):143-156 . PubMed Wang J et al. 2018. Cell. 176(1-2):334-347 . PubMed Snell LM, et al. 2018. Immunity. 49:678. PubMed Miyakoda M, et al. 2012. J Immunol. 189:4396. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Chen X, et al. 2021. Cell Rep. 37:109991. PubMed Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed Moore MJ et al. 2018. eLife. 7 pii: e33057. PubMed Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed Hartmann W, et al. 2020. Cell Reports. 29(8):2243-2256.e4.. PubMed Li M, et al. 2020. J Immunother Cancer. 8:00. PubMed\u003cbr\u003e\nRRID: AB_10639935 (BioLegend Cat. No. 125209) AB_10639727 (BioLegend Cat. No. 125210)\u003cbr\u003e\nStructure: Ig superfamily\u003cbr\u003e\nDistribution: T cells and NK cells, as well as T regulatory cells\u003cbr\u003e\nCell Type: NK cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Immunology, Inhibitory Molecules\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Baixeras E et al. 1992. J. Exp. Med. 176:327.\u003cbr\u003e\nGene ID: 16768\u003cbr\u003e\nUniProt: View information about CD223 on UniProt.org\u003cbr\u003e\nClone: C9B7W\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: LAG3, LAG-3\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862953513129,"sku":"125209","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-125209","provider":"Iright","version":"1.0","type":"link"}