{"product_id":"biolegend-126610","title":"Biolegend, 126610, PerCP\/Cyanine5.5 anti-mouse CD8b Antibody, 100μg","description":"\u003cp\u003eCD8b is the 32 kD β chain of CD8, also known as Lyt-3 or Ly-3. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse thymocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): McNical AM, et al. 2007. Eur. J. Immunol. 37:1634.\u003cbr\u003e\nProduct Citations: Lucca L, et al. 2014. J Immunol. 193:3267. PubMed Konjar S, et al. 2022. Proc Natl Acad Sci U S A. 119:e2202144119. PubMed Mackin S, et al. 2023. Nat Microbiol. 8:569. PubMed Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed Fiege JK, et al. 2021. Cell Host Microbe. 29:1815. PubMed Zhang W, et al. 2022. Dev Cell. 57:329. PubMed Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed Acker A, et al. 2014. PLoS One. 9:110015. PubMed Gimblet C et al. 2017. Cell host \u0026amp; microbe. 22(1):13-24 . PubMed Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed Jin RM, et al. 2017. J Immunol. 198:352. PubMed Hossain DMS, et al. 2018. J Clin Invest. 128:644. PubMed Hum NR, et al. 2022. Front Oncol. 12:928474. PubMed Panea C, et al. 2021. Commun Biol. 4:913. PubMed de Reuver R, et al. 2021. Cell Reports. 36(6):109500. PubMed LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed Hassan AO, et al. 2020. Cell. 183:169. PubMed Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed Gubin MM, et al. 2018. Cell. 175:1014. PubMed Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed Bennion BG, et al. 2019. J Virol. 93. PubMed Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed Sivaram N, et al. 2019. J Clin Invest. 130. PubMed Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed Wang Y, et al. 2022. Nat Cell Biol. 24:230. PubMed\u003cbr\u003e\nRRID: AB_961304 (BioLegend Cat. No. 126609) AB_2260149 (BioLegend Cat. No. 126610)\u003cbr\u003e\nStructure: Ig superfamily, CD8β chain, 34 kD\u003cbr\u003e\nDistribution: Majority of thymocytes, cytotoxic T cell subsets\u003cbr\u003e\nFunction: Co-receptor of TCR\u003cbr\u003e\nLigand\/Receptor: MHC class I\u003cbr\u003e\nCell Type: T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press. 2. Zamoyska R. 1994. Immunity 1:243-246. 3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523. 4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503.\u003cbr\u003e\nGene ID: 12526\u003cbr\u003e\nUniProt: View information about CD8beta on UniProt.org\u003cbr\u003e\nClone: YTS156.7.7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Lyt-3, Ly-3\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864108355753,"sku":"126610","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-126610","provider":"Iright","version":"1.0","type":"link"}