{"product_id":"biolegend-126628","title":"Biolegend, 126628, Alexa Fluor® 488 anti-mouse CD8b (Ly-3) Antibody, 100μg","description":"\u003cp\u003eCD8b is the 32 kD β chain of CD8, also known as Lyt-3 or Ly-3. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse thymocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality testedIHC-F - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg\/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): McNical AM, et al. 2007. Eur. J. Immunol. 37:1634.\u003cbr\u003e\nProduct Citations: Peymanfar Y, et al. 2023. Int J Mol Sci. 24:. PubMed Ozga AJ, et al. 2022. Immunity. 55:82. PubMed Blanas A, et al. 2022. iScience. 25:104719. PubMed van Loon K, et al. 2022. Cancers (Basel). 14:. PubMed\u003cbr\u003e\nRRID: AB_2800618 (BioLegend Cat. No. 126627) AB_2800619 (BioLegend Cat. No. 126628)\u003cbr\u003e\nStructure: Ig superfamily, CD8β chain, 34 kD\u003cbr\u003e\nDistribution: Majority of thymocytes, cytotoxic T cell subsets\u003cbr\u003e\nFunction: Co-receptor of TCR\u003cbr\u003e\nLigand\/Receptor: MHC class I\u003cbr\u003e\nCell Type: T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press. 2. Zamoyska R. 1994. Immunity 1:243-246. 3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523. 4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503.\u003cbr\u003e\nGene ID: 12526\u003cbr\u003e\nUniProt: View information about CD8beta on UniProt.org\u003cbr\u003e\nClone: YTS156.7.7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Lyt-3, Ly-3\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864107864233,"sku":"126628","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-126628","provider":"Iright","version":"1.0","type":"link"}