{"product_id":"biolegend-127105","title":"Biolegend, 127105, Alexa Fluor® 647 anti-mouse CD317 (BST2, PDCA-1) Antibody, 25μg","description":"\u003cp\u003eCD317, known as BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, or PDCA-1, is type II transmembrane glycoprotein with a molecular mass of 29-33 kD. It is predominantly expressed on Type I IFN-producing cells (IPCs) in naïve mice, but is up-regulated on most cell types following stimulation with type I IFNs and IFN-gamma. It is highly expressed on terminally differentiated normal plasmacytoid dendritic cells and some tumor cells, such as multiple myeloma, renal cell carcinoma, and melanoma cells. BST2 was recently identified as an IFN-induced cellular response factor that blocks release of HIV-1 and other retroviruses from infected cells. BST2 has been found to be the natural ligand of ILT7 in human model.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse plasmacytoid dendritic cells (DCs)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Blasius AL, et al. 2006. J. Immunol. 177:3260.\u003cbr\u003e\nProduct Citations: Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed Hirata SI, et al. 2020. Allergy. 75:1939. PubMed Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed\u003cbr\u003e\nRRID: AB_2228222 (BioLegend Cat. No. 127105) AB_2067120 (BioLegend Cat. No. 127106)\u003cbr\u003e\nStructure: Type II transmembrane glycoprotein with a molecular mass of 29-33 kD.\u003cbr\u003e\nDistribution: Expressed on type I IFN-producing cells, plasmacytoid dendritic cells, and neoplastic B cells, such as multiple myeloma.\u003cbr\u003e\nFunction: Recently identified as antiviral protein that blocks the release of nascent retrovirus or other particles from infected cells.\u003cbr\u003e\nCell Type: Dendritic cells, B cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity, Costimulatory Molecules\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Douglas JL, et al. 2009. J Virol. 83:7931.2. Cao W, et al. 2009. J. Exp. Med. 206:1603.3. Neil SJ, et al. 2008. Nature 451:425.\u003cbr\u003e\nGene ID: 69550\u003cbr\u003e\nUniProt: View information about CD317 on UniProt.org\u003cbr\u003e\nClone: 129C1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, PDCA-1\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860795183273,"sku":"127105","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-127105","provider":"Iright","version":"1.0","type":"link"}