{"product_id":"biolegend-127212","title":"Biolegend, 127212, Pacific Blue™ anti-mouse CD73 Antibody, 100μg","description":"\u003cp\u003eCD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b + myeloid cells. In spleen, it is largely expressed on T cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: CHO cells transfected with mouse CD73\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981. Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P)\u003cbr\u003e\nProduct Citations: Bergot AS, et al. 2020. J Immunol. 204:1787. PubMed Su W, et al. 2020. Cell Metabolism. 32(6):996-1011.e7. PubMed Garhyan J, et al. 2015. Am J Pathol. 185: 1924-1934. PubMed Chatterjee S et al. 2017. Cell metabolism. 27(1):85-100 . PubMed Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed Fang F, et al. 2021. Cell Rep. 37:109981. PubMed Graham JB, et al. 2017. Curr Protoc Mouse Biol. 7:221. PubMed\u003cbr\u003e\nRRID: AB_11218608 (BioLegend Cat. No. 127211) AB_11219190 (BioLegend Cat. No. 127212)\u003cbr\u003e\nStructure: 69 kD GPI-anchored surface protein\u003cbr\u003e\nDistribution: Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73\u003cbr\u003e\nFunction: A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates\u003cbr\u003e\nLigand\/Receptor: Nucleoside 5' monophosphate\u003cbr\u003e\nCell Type: B cells, Mesenchymal Stem Cells, Tregs\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981. 2. Resta R, et al. 1998. Immunol. Rev. 161:95.\u003cbr\u003e\nRegulation: Expression is regulated by the development and maturation of leukocytes\u003cbr\u003e\nGene ID: 23959\u003cbr\u003e\nUniProt: View information about CD73 on UniProt.org\u003cbr\u003e\nClone: TY\/11.8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Ecto-5'-nucleotidase\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864061825193,"sku":"127212","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-127212","provider":"Iright","version":"1.0","type":"link"}