{"product_id":"biolegend-127813","title":"Biolegend, 127813, PerCP\/Cyanine5.5 anti-mouse TCR Vα2 Antibody, 25μg","description":"\u003cp\u003eThe TCR alpha (α) chain complexes with the TCR beta (β) chain to form the T cell receptor in 95% of T cells, whereas the remaining 5% of T cells express gamma and delta chains (γ\/δ).  TCR Vα2 is a distinct TCR subfamily found in mice having the a, b, and c haplotypes.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Soluble TCR from mouse CTL clone KB5-C20\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: The B20.1 antibody recognizes most members of the Va2 TCR subfamily in mice having the a, b, and c haplotypes.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Pircher H, et al. 1992. Eur. J. Immunol.. 22:399. Gregoire C, et al. 1991. P. Natl. Acad. Sci. USA 88:8077. Kao C, et al. 2005. Int. Immunol.17:1607. PubMed Steptoe RJ, et al. 2007. J. Immunol. 178:2094. PubMed Rao RR, et al. 2012. Immunity. 36:374. PubMed.\u003cbr\u003e\nProduct Citations: Zenke S, et al. 2022. Nat Commun. 13:6459. PubMed Ooi JD, et al. 2017. Nature. 545:243. PubMed Caronni N, et al. 2018. Cancer Res. 78:1685. PubMed Pauken KE, et al. 2020. Cell Reports. 31(13):107827. PubMed LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed\u003cbr\u003e\nRRID: AB_1186118 (BioLegend Cat. No. 127813) AB_1186116 (BioLegend Cat. No. 127814)\u003cbr\u003e\nDistribution: On a subset of T cells\u003cbr\u003e\nFunction: In combination of V β, Vα recognizes MHC\/peptide complex on the antigen-presenting cells.\u003cbr\u003e\nInteraction: Associate with Vβ and CD3 complex to form TCR complexes expressing on cell surface.\u003cbr\u003e\nLigand\/Receptor: TCR recognizes MHC\/peptide complex\u003cbr\u003e\nCell Type: T cells\u003cbr\u003e\nBiology Area: Adaptive Immunity, Immunology\u003cbr\u003e\nMolecular Family: TCRs\u003cbr\u003e\nAntigen References: 1. Kubo RT, et al. 1989. J. Immunol.. 142:2736. 2. Pircher H, et al. 1992. Eur. J. Immunol. 22:399.\u003cbr\u003e\nGene ID: 21473\u003cbr\u003e\nUniProt: View information about TCR Valpha2 on UniProt.org\u003cbr\u003e\nClone: B20.1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T cell receptor Vα 2\u003cbr\u003e\nIsotype: Rat IgG2a, λ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862548992169,"sku":"127813","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-127813","provider":"Iright","version":"1.0","type":"link"}