{"product_id":"biolegend-127816","title":"Biolegend, 127816, Pacific Blue™ anti-mouse TCR Vα2 Antibody, 100μg","description":"\u003cp\u003eThe TCR alpha (α) chain complexes with the TCR beta (β) chain to form the T cell receptor in 95% of T cells, whereas the remaining 5% of T cells express gamma and delta chains (γ\/δ).  TCR Vα2 is a distinct TCR subfamily found in mice having the a, b, and c haplotypes.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Soluble TCR from mouse CTL clone KB5-C20\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: The B20.1 antibody recognizes most members of the Va2 TCR subfamily in mice having the a, b, and c haplotypes.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Pircher H, et al. 1992. Eur. J. Immunol.. 22:399. Gregoire C, et al. 1991. P. Natl. Acad. Sci. USA 88:8077. Kao C, et al. 2005. Int. Immunol.17:1607. PubMed Steptoe RJ, et al. 2007. J. Immunol. 178:2094. PubMed Rao RR, et al. 2012. Immunity. 36:374. PubMed.\u003cbr\u003e\nProduct Citations: Dölz M, et al. 2022. iScience. 25:105372. PubMed Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed\u003cbr\u003e\nRRID: AB_10612927 (BioLegend Cat. No. 127815) AB_10613647 (BioLegend Cat. No. 127816)\u003cbr\u003e\nDistribution: On a subset of T cells\u003cbr\u003e\nFunction: In combination of V β, Vα recognizes MHC\/peptide complex on the antigen-presenting cells.\u003cbr\u003e\nInteraction: Associate with Vβ and CD3 complex to form TCR complexes expressing on cell surface.\u003cbr\u003e\nLigand\/Receptor: TCR recognizes MHC\/peptide complex\u003cbr\u003e\nCell Type: T cells\u003cbr\u003e\nBiology Area: Adaptive Immunity, Immunology\u003cbr\u003e\nMolecular Family: TCRs\u003cbr\u003e\nAntigen References: 1. Kubo RT, et al. 1989. J. Immunol.. 142:2736. 2. Pircher H, et al. 1992. Eur. J. Immunol. 22:399.\u003cbr\u003e\nGene ID: 21473\u003cbr\u003e\nUniProt: View information about TCR Valpha2 on UniProt.org\u003cbr\u003e\nClone: B20.1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T cell receptor Vα 2\u003cbr\u003e\nIsotype: Rat IgG2a, λ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862548697257,"sku":"127816","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-127816","provider":"Iright","version":"1.0","type":"link"}