{"product_id":"biolegend-129709","title":"Biolegend, 129709, Alexa Fluor® 647 anti-mouse CD199 (CCR9) Antibody, 25μg","description":"\u003cp\u003eCCR9 is a member of the G protein coupled receptor family and is involved in T cell development in the thymus and in gut-associated immune response. Mouse CCR9 is highly expressed on different stages of thymocytes and upregulated on CD4+CD8+ cells. It is expressed to a much lower level on spleen and lymph node cells. Expression of CCR9 on γ\/δ T cells in the intraepithelial and small intestine has been reported. The interaction of CCR9 with its ligand CCL25 (TECK, thymus-expressed chemokine) may direct the trafficking of developing T cells in the thymus and generation of gut-specific immunological memory.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nProduct Citations: Lo WL, et al. 2023. Nat Immunol. 24:676. PubMed Ersching J, et al. 2016. PLoS Pathog. 12: 1005698. PubMed\u003cbr\u003e\nRRID: AB_2073248 (BioLegend Cat. No. 129709) AB_2073247 (BioLegend Cat. No. 129710)\u003cbr\u003e\nStructure: A membrane of G-protein coupled receptors and contains seven transmembrain domains. Mouse CCR9 has above 80% amino acid sequence homology with human counterpart.\u003cbr\u003e\nDistribution: CD4+CD8+ thymocytes, γ\/δ T cells\u003cbr\u003e\nInteraction: CCL25\u003cbr\u003e\nLigand\/Receptor: CCL25\u003cbr\u003e\nCell Type: T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Zaballos A, et al. 1999. J. Immunol. 162:56712. Wurbel MA, et al. 2007. J. Immunol. 178:75983. Wurbel MA, et al. 2006. Eur. J. Immunol. 36:73\u003cbr\u003e\nGene ID: 12769\u003cbr\u003e\nUniProt: View information about CD199 on UniProt.org\u003cbr\u003e\nClone: 9B1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: GPR 9-6, CDw199, Chemokine Receptor 9\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862502527145,"sku":"129709","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-129709","provider":"Iright","version":"1.0","type":"link"}