{"product_id":"biolegend-129807","title":"Biolegend, 129807, Alexa Fluor® 647 anti-mouse CD196 (CCR6) Antibody, 25μg","description":"\u003cp\u003eCCR6 is a G-protein linked chemokine receptor which binds the chemokine CCL20\/MIP-3a. It is expressed on B lymphocytes and certain subsets of dendritic cells and T cells. CCR6 is reported to be involved in mucosal immune response and lymphocyte migration and homeostasis.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: N-terminal peptide (aa. 2-38) of mouse CCR6 fused with GST\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nProduct Citations: Matter AL, et al. 2022. J Immunol. 209:1880. PubMed Wang L, et al. 2019. Cell Rep. 29:1848. PubMed Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed Shen X, et al. 2021. Front Immunol. 12:710750. PubMed Nguyen HD, et al. 2020. Cell Rep. 33:108316. PubMed\u003cbr\u003e\nRRID: AB_1227498 (BioLegend Cat. No. 129807) AB_1227497 (BioLegend Cat. No. 129808)\u003cbr\u003e\nStructure: G-protein linked, seven transmembrane, domain-spanning chemokine receptor; 367 amino acids long and about 75% homology with human CCR6\u003cbr\u003e\nDistribution: Expressed on spleen B cells, immature splenic dendritic cells in Peyer's patches, and some T cell subset\u003cbr\u003e\nFunction: Important in regulating mucosal immune response\u003cbr\u003e\nLigand\/Receptor: CCL20\/MIP-3a\u003cbr\u003e\nCell Type: B cells, Dendritic cells, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Rossi D, et al. 1997. J. Immunol. 158:1033. 2. Lukacs N, et al. 2001. J. Exp. Med. 194:551. 3. Cook DN, et al. 2000. Immunity 12:495\u003cbr\u003e\nGene ID: 12458\u003cbr\u003e\nUniProt: View information about CD196 on UniProt.org\u003cbr\u003e\nClone: 29-2L17\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD196, BN-1, CKR-L3, DRY6, KY411, STRL22\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860885819561,"sku":"129807","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-129807","provider":"Iright","version":"1.0","type":"link"}