{"product_id":"biolegend-132006","title":"Biolegend, 132006, PE anti-mouse CD226 (DNAM-1) Antibody, 100μg","description":"\u003cp\u003eCD226 (DNAM-1) is constitutively expressed on native CD8+ cells and on some CD4+ T cells, macrophages and NK cells. CD226 is involved in NK and T cell mediated cytotoxicity against certain tumors. CD155 and CD112 are the ligands for CD226. This antibody (480.1) is reported by the developer to partially block the binding of mouse CD155.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Jurkat cells transfected with mouse CD226\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Dardalhon V, et al. 2005. J. Immunol. 175:1558\u003cbr\u003e\nProduct Citations: He Y, et al. 2017. J Autoimmun. 10.1016\/j.jaut.2017.04.001. PubMed Victorino F, et al. 2021. eLife. 0.416666666666667. PubMed\u003cbr\u003e\nRRID: AB_1279173 (BioLegend Cat. No. 132006)\u003cbr\u003e\nStructure: 65 kd glycoprotein of a member of Ig-superfamily containing 2 immunologlobulin-like domains.\u003cbr\u003e\nDistribution: Expressed on majority of CD8+ and CD4+ T cells, NK cells, monocytes\/macrophages and a subset of B cells. Reported to upregulated on Th1 cells, but downregulated in Th2 cells.\u003cbr\u003e\nFunction: Mediates cellular adhesion, involved in LFA-1 co-stimulatory signal for T cell differentiation and proliferation\u003cbr\u003e\nLigand\/Receptor: CD112 and CD155\u003cbr\u003e\nCell Type: B cells, Macrophages, Monocytes, NK cells, T cells, Th1\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Tahara Hanaoka S et al. 2006 Blood 107:1491 2. Shibuya K et al. 2003. J. Exp. Med. 198:1829\u003cbr\u003e\nGene ID: 225825\u003cbr\u003e\nUniProt: View information about CD226 on UniProt.org\u003cbr\u003e\nClone: 480.1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: DNAM-1, PTA1(platelet and T cell activation antigen 1), TLISA1, LFA-1 associated Molecule PTA-1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862406680745,"sku":"132006","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-132006","provider":"Iright","version":"1.0","type":"link"}