{"product_id":"biolegend-133305","title":"Biolegend, 133305, Pacific Blue™ anti-mouse Lineage Cocktail with Isotype Ctrl, 25tests","description":"\u003cp\u003eThe mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes\/macrophages, granulocytes, NK cells, and erythrocytes. The Pacific Blue™ Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3, clone 17A2; anti-mouse Ly-6G\/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1\/70; anti-mouse CD45R\/B220, clone RA3-6B2; anti-mouse TER-119\/Erythroid cells, clone Ter-119. Pacific Blue™ Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin.  Materials provided: 1. Pacific Blue™ anti-mCD3\/ Pacific Blue™ anti-mLy-6G(Ly-6C)\/ Pacific Blue™ anti-mCD11b\/ Pacific Blue™ anti-mCD45R(B220)\/ Pacific Blue™ anti-mTer-119 2. Pacific Blue™ Rat IgG2b\/Rat IgG2a isotype controls   Materials not provided: Cell Staining Buffer (Cat. No. 420201)\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: This two-reagent set contains Pacific Blue™ conjugated antibodies and isotype controls in optimal concentration for flow cytometric analysis.\u003cbr\u003e\nPreparation: The antibodies are purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solutions should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nProduct Citations: Palakurthi B, et al. 2023. Nat Commun. 14:2109. PubMed Zhuang R, et al. 2022. Circ Res. 1662:130. PubMed Tomiyama E, et al. 2022. Br J Cancer. 1312:127. PubMed Ahrends T, et al. 2021. Cell. 184:5715. PubMed Garama D, et al. 2015. Mol Cell Biol. 35: 3646 - 3656. PubMed Sztwiertnia I, et al. 2020. PLoS One. 15:e0233789. PubMed Ding P, et al. 2022. Bone Res. 10:42. PubMed Grayson MH, et al. 2018. Front Immunol. 9:1587. PubMed\u003cbr\u003e\nRRID: AB_11126168 (BioLegend Cat. No. 133305) AB_11126978 (BioLegend Cat. No. 133306)\u003cbr\u003e\nDistribution: T cells, B cells, monocytes\/macrophages, granulocytes, NK cells, and erythrocytes\u003cbr\u003e\nCell Type: B cells, Erythrocytes, Granulocytes, Macrophages, Monocytes, NK cells, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nAntigen References: 1. Morrison SJ, et al. 1997. Development 124:1929. 2. Okada S, et al. 1992. Blood 80:3044. 3. Spangrude GJ, et al. 1988. Science 241:58. 4. Spangrude GJ, et al. 1990. Exp. Hematol. 18:920. 5. Tan SL, et al. 2006. J. Immunol. 176:2872.\u003cbr\u003e\nGene ID: 104231\u003cbr\u003e\nUniProt: View information about Lineage on UniProt.org\u003cbr\u003e\nClone: 17A2; RB6-8C5; RA3-6B2; Ter-119; M1\/70;\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Lin-1\u003cbr\u003e\nIsotype: Rat IgG2a\/Rat IgG2b\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860826181801,"sku":"133305","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-133305","provider":"Iright","version":"1.0","type":"link"}