{"product_id":"biolegend-134003","title":"Biolegend, 134003, PE anti-mouse CD366 (Tim-3) Antibody, 50μg","description":"\u003cp\u003eCD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b + macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: mTim-3 protein\/Freund adjuvant\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clone B8.2C12 only binds to the BALB\/c allele of Tim-3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed\u003cbr\u003e\nProduct Citations: Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed Li H, et al. 2022. Cancer Cell. 40:36. PubMed Shallberg LA, et al. 2022. PLoS Pathog. 18:e1010296. PubMed Wei M, et al. 2022. Front Immunol. 13:1017574. PubMed Chen X, et al. 2022. J Extracell Vesicles. 11:e12279. PubMed Edwards CL, et al. 2023. J Clin Invest. 133: . PubMed Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed Xie A et al. 2017. Endocrinology. 158(10):3140-3151 . PubMed Zhu H, et al. 2018. Elife. 7:. PubMed Zuo S, et al. 2021. J Immunother Cancer. 9:. PubMed Zhang X, et al. 2020. J Cancer. 11:648. PubMed Zhong M, et al. 2022. Cell Death Dis. 13:671. PubMed Puleston D, et al. 2014. Elife. 3:3706. PubMed Song X, et al. 2022. Transl Oncol. 15:101306. PubMed\u003cbr\u003e\nRRID: AB_1626181 (BioLegend Cat. No. 134003) AB_1626177 (BioLegend Cat. No. 134004)\u003cbr\u003e\nStructure: Transmembrane protein containing immunoglobulin domain and mucin-like domain. It can exist as a soluble form lacking mucin and transmembrane domains.\u003cbr\u003e\nDistribution: Expressed on activated Th1 lymphocytes, CD11b+ macrophages, and dendritic cells.\u003cbr\u003e\nFunction: Play a role in regulating macrophage activation, T cell apoptosis and immune tolerance.\u003cbr\u003e\nLigand\/Receptor: Galectin -9\u003cbr\u003e\nCell Type: Dendritic cells, Macrophages, Th1\u003cbr\u003e\nBiology Area: Immunology, Inhibitory Molecules\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102 2. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454 3. Mooney L, et al. 2002. Nature. 415:536 4. Rodriguez-Manzanet R, et al. 2009. Immunol. Rev. 229(1):259\u003cbr\u003e\nGene ID: 171285\u003cbr\u003e\nUniProt: View information about CD366 on UniProt.org\u003cbr\u003e\nClone: B8.2C12\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862896922793,"sku":"134003","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-134003","provider":"Iright","version":"1.0","type":"link"}