{"product_id":"biolegend-135016","title":"Biolegend, 135016, PE\/Cyanine5 anti-mouse CD127 (IL-7Rα) Antibody, 200μg","description":"\u003cp\u003eCD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4\/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.\u003cbr\u003e\n200μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: IL-7Ra-IgG1 fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE\/Cyanine5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.15 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clone A7R34 is able to completely block the binding of clone S18006K to IL-7Rα. It does not block SB\/199.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. Hashi H, et al. 2001. J. Immunol. 166:3702. Taylor R, et al. 2007. J. Immunol. 178:5659. Mazzon C, et al. 2011. Blood. 118:2733. PubMed Jin J, et al. 2011. J. Immunol. doi:10.4049\/jimmunol.1001238. PubMed\u003cbr\u003e\nProduct Citations: Alam A, et al. 2022. Cancer Cell. 40:153. PubMed Moudra A, et al. 2021. J Immunol. 206:2109. PubMed Browning LM, et al. 2020. Cell Rep. 33:108219. PubMed Peng X, et al. 2014. J Immunol. 193:1268. PubMed Mann M, et al. 2018. Cell Rep. 25:2992. PubMed Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed Green CD et al. 2018. Developmental cell. 46(5):651-667 . PubMed Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed Teng F, et al. 2021. Cell Rep. 37:110051. PubMed\u003cbr\u003e\nRRID: AB_1937262 (BioLegend Cat. No. 135015) AB_1937261 (BioLegend Cat. No. 135016)\u003cbr\u003e\nStructure: Type I transmembrane glycoprotein, associate with CD132, 60-90 kD\u003cbr\u003e\nDistribution: Immature B cells through early pre-B stage, thymocytes (except CD4\/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells\u003cbr\u003e\nFunction: T cell and immature B cell proliferation and development\u003cbr\u003e\nLigand\/Receptor: IL-7\u003cbr\u003e\nCell Type: B cells, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. 2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246. 3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.\u003cbr\u003e\nGene ID: 16197\u003cbr\u003e\nUniProt: View information about CD127 on UniProt.org\u003cbr\u003e\nClone: A7R34\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IL-7 receptor α chain, IL-7Rα\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862524645545,"sku":"135016","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-135016","provider":"Iright","version":"1.0","type":"link"}