{"product_id":"biolegend-135206","title":"Biolegend, 135206, PE anti-mouse CD279 (PD-1) Antibody, 200μg","description":"\u003cp\u003eCD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.\u003cbr\u003e\n200μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: PD-1 cDNA followed by PD-1-Ig fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue3, in vivo blocking of PD-1 binding to its ligands2,3, and spatial biology (IBEX)5,6.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed Lázár-Molnár E, et al. 2008. Proc. Natl. Acad. Sci. USA 105:2658. (Block) Liang SC, et al. 2003. Eur. J. Immunol. 33:2706. (FC, IHC, Block) Tobias J, et al. 2020. Front Immunol. 11:895 (FC, ELISA) PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed Spitsin S, et al. 2012. Clin Vaccine Immunol. 1.313888889. PubMed Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed Hamdan F, et al. 2023. Mol Ther Oncolytics. 28:264. PubMed Florian DC, et al. 2023. Cancer Res Commun. 3:223. PubMed Gao Y, et al. 2023. iScience. 26:106729. PubMed Nishi W, et al. 2023. Nat Commun. 14:3157. PubMed Yang QC, et al. 2023. iScience. 26:106916. PubMed Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed Serra M, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:609. PubMed Liu M, et al. 2022. Cells. 11: . PubMed Wei M, et al. 2022. Front Immunol. 13:1017574. PubMed Zhao X, et al. 2022. STAR Protoc. 3:101859. PubMed Domingos-Pereira S, et al. 2022. Int J Mol Sci. 24: . PubMed Wei J, et al. 2022. Cancer Prev Res (Phila). 15:285. PubMed Wight AE, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200757119. PubMed Renrick AN, et al. 2021. Front Immunol. 607044:12. PubMed Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed Lin YN, et al. 2022. Oncoimmunology. 11:2027136. PubMed Zhang R, et al. 2021. Cell Mol Immunol. 18:1222. PubMed Hamilton JAG, et al. 2021. Aging Cell. 20:e13309. PubMed Kim CJ, et al. 2018. Immunity. 49:1034. PubMed Sasse C, et al. 2022. Pathogens. 11:. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Stolp B, et al. 2022. Cell Rep. 38:110387. PubMed Feizi N, et al. 2021. Cell Death Dis. 12:1026. PubMed Lu SX, et al. 2021. Cell. . PubMed Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed Kummer MP, et al. 2021. EMBO J. 40:e108662. PubMed Cai B, et al. 2021. Mol Cancer. 20:165. PubMed Kramer KJ, et al. 2022. Nat Commun. 13:3466. PubMed Lv H, et al. 2020. Cell Metabolism. 33(1):110-127.e5. PubMed Gary EN, et al. 2021. iScience. 24(7):102699. PubMed Hu J, et al. 2020. Biomed Pharmacother. 127:110229. PubMed Russell SL, et al. 2019. Cell Rep. 29:3564. PubMed Jing L, et al. 2022. Front Immunol. 13:864995. PubMed Wang Z, et al. 2021. J Virol. 95:e0141421. PubMed Zhang X, et al. 2021. Front Immunol. 12:680068. PubMed Domeier P, et al. 2016. J Exp Med. 213: 715 - 732. PubMed Soni C, et al. 2014. J Immunol. 193:4400. PubMed Liu J, et al. 2019. J Exp Med. 216:656. PubMed Wang GZ, et al. 2019. Nat Commun. 10:1125. PubMed Zhang P, et al. 2022. BMC Med. 20:435. PubMed Mintz MA, et al. 2019. Immunity. 51:310. PubMed Ekeke CN, et al. 2020. J Thorac Cardiovasc Surg. S0022-5223:33337. PubMed Zhang YN, et al. 2020. EBioMedicine. 56:102819. PubMed Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed Fortner K, et al. 2017. Journal of Autoimmunity. 10.1016\/j.jaut.2017.05.003. PubMed Wei W, et al. 2022. mSystems. 7:e0046922. PubMed Zuo S, et al. 2021. J Immunother Cancer. 9:. PubMed Reinfeld BI, et al. 2021. Nature. 593:282. PubMed Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed Mittal A, et al. 2021. Sci Rep. 11:10731. PubMed Zhang X, et al. 2020. Endocr Relat Cancer. 27:469. PubMed Pham D, et al. 2019. Cell Rep. 29:1203. PubMed Beloor J et al. 2018. Cell host \u0026amp; microbe. 23(4):549-556 . PubMed Schell S, et al. 2017. J Immunol. 10.4049\/jimmunol.1700611. PubMed Chen C, et al. 2021. J Virol. 95:e0082921. PubMed Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed Zhao X, et al. 2022. iScience. 25:104690. PubMed Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed Wong E, et al. 2015. J Immunol. 194:4130. PubMed Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed Fang W, et al. 2021. J Exp Clin Cancer Res. 40:4. PubMed Wagner AK, et al. 2022. iScience. 25:105137. PubMed Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed Hamdan F, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(8):. PubMed Roberto MP, et al. 2021. Immunity. 54(8):1807-1824.e14. PubMed Zhang Z, et al. 2022. J Inflamm Res. 15:5595. PubMed Fusciello M, et al. 2019. Nat Commun. 4.407638889. PubMed Li X, et al. 2021. Front Immunol. 12:779560. PubMed\u003cbr\u003e\nRRID: AB_1877232 (BioLegend Cat. No. 135205) AB_1877231 (BioLegend Cat. No. 135206)\u003cbr\u003e\nStructure: A 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily.\u003cbr\u003e\nDistribution: Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.\u003cbr\u003e\nFunction: Involved in lymphocyte clonal selection and peripheral tolerance, prolonged survival of allografts.\u003cbr\u003e\nLigand\/Receptor: B7-H1 (PD-L1) and B7-DC (PD-L2)\u003cbr\u003e\nCell Type: B cells, T cells\u003cbr\u003e\nBiology Area: Cancer Biomarkers, Immunology, Inhibitory Molecules\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Nishimura H, et al. 2001. Science 291:319 2. Agata Y, et al. 1996. Int. Immunol. 8:765 3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706 4. Barber DL, et al. 2006. Nature 439:682 5. Keir ME, et al. 2005. J. Immunol. 175:7372 6. Koehn BH. et al. 2008. J Immunol. 181:5313\u003cbr\u003e\nGene ID: 18566\u003cbr\u003e\nUniProt: View information about CD279 on UniProt.org\u003cbr\u003e\nClone: 29F.1A12\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PD-1, Programmed Death-1, PDCD1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866548097193,"sku":"135206","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-135206","provider":"Iright","version":"1.0","type":"link"}