{"product_id":"biolegend-135208","title":"Biolegend, 135208, PerCP\/Cyanine5.5 anti-mouse CD279 (PD-1) Antibody, 100μg","description":"\u003cp\u003eCD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: PD-1 cDNA followed by PD-1-Ig fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue3, in vivo blocking of PD-1 binding to its ligands2,3, and spatial biology (IBEX)5,6.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed Lázár-Molnár E, et al. 2008. Proc. Natl. Acad. Sci. USA 105:2658. (Block) Liang SC, et al. 2003. Eur. J. Immunol. 33:2706. (FC, IHC, Block) Tobias J, et al. 2020. Front Immunol. 11:895 (FC, ELISA) PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Du J, et al. 2022. Sci Immunol. 7:eabo5407. PubMed Lo WL, et al. 2023. Nat Immunol. 24:676. PubMed Otano I, et al. 2021. Nat Commun. 12:7296. PubMed Hansel C, et al. 2019. Front Pharmacol. 10:244. PubMed Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed Lovisa S, et al. 2020. Sci Signal. 13:00. PubMed Utz B, et al. 2020. Sci Rep. 10:18733. PubMed Gozgit JM, et al. 2021. Cancer Cell. :. PubMed Pascual–García M, et al. 2019. Nat Commun. 10:2416. PubMed Haikala HM, et al. 2019. Nat Commun. 10:620. PubMed Rashid MH, et al. 2021. Oncol Rep. 45:1171. PubMed Wu L, et al. 2020. Cell Rep. 33:108327. PubMed Hekim C, et al. 2017. Cancer Immunol Res. 5:157. PubMed Barbet G, et al. 2018. Immunity. 48:584. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Rousso-Noori L, et al. 2021. Nat Commun. 12:3615. PubMed Truckenbrod EN, et al. 2021. Elife. 10:. PubMed Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed Stienne C, et al. 2022. Cell Rep. 38:110553. PubMed Guiteras J, et al. 2022. Int J Mol Sci. 23:. PubMed\u003cbr\u003e\nRRID: AB_10550092 (BioLegend Cat. No. 135207) AB_2159184 (BioLegend Cat. No. 135208)\u003cbr\u003e\nStructure: A 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily.\u003cbr\u003e\nDistribution: Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.\u003cbr\u003e\nFunction: Involved in lymphocyte clonal selection and peripheral tolerance, prolonged survival of allografts.\u003cbr\u003e\nLigand\/Receptor: B7-H1 (PD-L1) and B7-DC (PD-L2)\u003cbr\u003e\nCell Type: B cells, T cells\u003cbr\u003e\nBiology Area: Cancer Biomarkers, Immunology, Inhibitory Molecules\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Nishimura H, et al. 2001. Science 291:319 2. Agata Y, et al. 1996. Int. Immunol. 8:765 3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706 4. Barber DL, et al. 2006. Nature 439:682 5. Keir ME, et al. 2005. J. Immunol. 175:7372 6. Koehn BH. et al. 2008. J Immunol. 181:5313\u003cbr\u003e\nGene ID: 18566\u003cbr\u003e\nUniProt: View information about CD279 on UniProt.org\u003cbr\u003e\nClone: 29F.1A12\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PD-1, Programmed Death-1, PDCD1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866549047465,"sku":"135208","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-135208","provider":"Iright","version":"1.0","type":"link"}