{"product_id":"biolegend-135310","title":"Biolegend, 135310, APC anti-mouse CD135 Antibody, 100μg","description":"\u003cp\u003eCD135, also known as Flk-2, Flt3, and Ly-72, is a type III tyrosine kinase receptor. It is expressed on early B lymphoid lineage cells in bone marrow, on primitive myeloid progenitors within the BM CD34+ cell population. Ligation of Flk-2 with Flt3 ligand regulates the growth of hematopoietic stem cells and promotes the survival of primitive hematopoietic progenitor cells with myeloid as well as B lymphoid potential. It was reported that the receptor tyrosine kinase Flt3 is required for dendritic cell development. Combined signaling through interleukin-7 receptors and Flt3 selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse Flt3 transfected cell line\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Sergejeva S, et al. 2004. Blood 103:1270. Auffray C, et al. 2009. J. Exp. Med. 206:595. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed\u003cbr\u003e\nProduct Citations: Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed Han X, et al. 2023. J Clin Invest. 133:. PubMed van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed Song R, et al. 2022. Immunology. 165:274. PubMed Tu J, et al. 2023. Ann Rheum Dis. 82:198. PubMed Zhu J, et al. 2022. Nat Commun. 13:7466. PubMed Jabbar S, et al. 2022. Exp Hematol Oncol. 11:83. PubMed Mitroulis I et al. 2018. Cell. 172(1-2):147-161 . PubMed Mann M, et al. 2018. Cell Rep. 25:2992. PubMed Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed Inoue D, et al. 2021. Nat Genet. 53:707. PubMed Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed Schlager S, et al. 2015. J Leukoc Biol. 98: 837 - 850. PubMed Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed McAlpine CS, et al. 2021. Nature. 595:701. PubMed Jia W, et al. 2021. Nat Commun. 12:2118. PubMed Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed Gao S, et al. 2022. Cell Res. 32:38. PubMed Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed Jonas B, et al. 2016. PLoS One. 11: 0159189. PubMed Ding P, et al. 2022. Bone Res. 10:42. PubMed Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed Wuggenig P, et al. 2020. Commun Biol. 3:130. PubMed Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed Oppezzo A, et al. 2020. J Clin Invest. 130:1377. PubMed Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed Li Z, et al. 2017. Sci Rep. 7:40380. PubMed Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed Giridhar P, et al. 2016. J Immunol. 197(11):4228-4239. PubMed Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed\u003cbr\u003e\nRRID: AB_1953264 (BioLegend Cat. No. 135309) AB_2107050 (BioLegend Cat. No. 135310)\u003cbr\u003e\nStructure: A 135-150 kD molecule belonging to the tyrosine kinase receptor family.\u003cbr\u003e\nDistribution: Expressed on early B lymphoid lineage cells in juvenile and adult bone marrow, primitive myeloid progenitors within the BM CD34+ cell population.\u003cbr\u003e\nFunction: Regulate the growth of hematopoietic stem cells and promote the survival of primitive hematopoietic progenitor cells.\u003cbr\u003e\nLigand\/Receptor: Flk-2\/FLT3 ligand\u003cbr\u003e\nCell Type: B cells, Hematopoietic stem and progenitors\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Waskow C, et al.Nat. Immunol. 9:676 2. Veiby OP, et al. 1996. Blood 88(4):1256 3. Veiby OP, et al. 1996. J. Immunol. 157(7):2953 4. Mattews W, et al. 1991. Cell. 65(7):1143 5. Hannum C, et al. 1994. Nature 368(2):643 6. Ogawa M, et al. 1998. Exp Hematol. 26(6):478\u003cbr\u003e\nGene ID: 14255\u003cbr\u003e\nUniProt: View information about CD135 on UniProt.org\u003cbr\u003e\nClone: A2F10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Flk-2, Flt3, Ly-72\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862521925801,"sku":"135310","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-135310","provider":"Iright","version":"1.0","type":"link"}