{"product_id":"biolegend-135324","title":"Biolegend, 135324, KIRAVIA Blue 520™ anti-mouse CD135 Antibody, 100μg","description":"\u003cp\u003eCD135, also known as Flk-2, Flt3, and Ly-72, is a type III tyrosine kinase receptor. It is expressed on early B lymphoid lineage cells in bone marrow, on primitive myeloid progenitors within the BM CD34+ cell population. Ligation of Flk-2 with Flt3 ligand regulates the growth of hematopoietic stem cells and promotes the survival of primitive hematopoietic progenitor cells with myeloid as well as B lymphoid potential. It was reported that the receptor tyrosine kinase Flt3 is required for dendritic cell development. Combined signaling through interleukin-7 receptors and Flt3 selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse Flt3 transfected cell line\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with KIRAVIA Blue 520™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * KIRAVIA Blue 520™ has an excitation maximum of 495 nm, and a maximum emission of 520 nm. KIRAVIA Blue™ 520 is a trademark of Sony. This product is subject to proprietary rights of Sony and is made and sold under license from Sony Corporation. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.Sony and the Sony logo are registered trademarks of Sony Corporation.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Sergejeva S, et al. 2004. Blood 103:1270. Auffray C, et al. 2009. J. Exp. Med. 206:595. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed\u003cbr\u003e\nRRID: AB_3068160 (BioLegend Cat. No. 135323) AB_3068160 (BioLegend Cat. No. 135324)\u003cbr\u003e\nStructure: A 135-150 kD molecule belonging to the tyrosine kinase receptor family.\u003cbr\u003e\nDistribution: Expressed on early B lymphoid lineage cells in juvenile and adult bone marrow, primitive myeloid progenitors within the BM CD34+ cell population.\u003cbr\u003e\nFunction: Regulate the growth of hematopoietic stem cells and promote the survival of primitive hematopoietic progenitor cells.\u003cbr\u003e\nLigand\/Receptor: Flk-2\/FLT3 ligand\u003cbr\u003e\nCell Type: B cells, Hematopoietic stem and progenitors\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Waskow C, et al.Nat. Immunol. 9:676 2. Veiby OP, et al. 1996. Blood 88(4):1256 3. Veiby OP, et al. 1996. J. Immunol. 157(7):2953 4. Mattews W, et al. 1991. Cell. 65(7):1143 5. Hannum C, et al. 1994. Nature 368(2):643 6. Ogawa M, et al. 1998. Exp Hematol. 26(6):478\u003cbr\u003e\nGene ID: 14255\u003cbr\u003e\nUniProt: View information about CD135 on UniProt.org\u003cbr\u003e\nClone: A2F10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Flk-2, Flt3, Ly-72\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862522024105,"sku":"135324","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-135324","provider":"Iright","version":"1.0","type":"link"}