{"product_id":"biolegend-135813","title":"Biolegend, 135813, PerCP\/Cyanine5.5 anti-mouse CD88 (C5aR) Antibody, 100μg","description":"\u003cp\u003eCD88 is a single chain protein with seven membrane-spanning regions and a molecular weight of 43 kD. CD88 is expressed by neutrophils, eosinophils, and monocytes. There have been reports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells, cardiomyocytes, and vascular endothelial cells. CD88 is coupled to heterotrimeric G proteins such as G i , and after C5a binding, the signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA 2 , among other molecules, resulting in chemotaxis, granule enzyme release, and superoxide anion production.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: mC5aR transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications for the relevant formats include: blocking of C5a binding.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Godau J, et al. 2004. J. Immunol. 173:3437. Shushakova N, et al. 2002. J. Clin. Invest. 110:1823. Kim SH, et al. 2013. Int Immunol. 25:623. PubMed\u003cbr\u003e\nProduct Citations: Bohrer AC, et al. 2022. Cell Rep. 40:111144. PubMed Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed\u003cbr\u003e\nRRID: AB_2750208 (BioLegend Cat. No. 135812) AB_2750209 (BioLegend Cat. No. 135813)\u003cbr\u003e\nStructure: Single chain protein with seven membrane-spanning regions, 43 kD.\u003cbr\u003e\nDistribution: Neutrophils, eosinophils, monocytes, glial cells, cerebellar granule cells, cardiomyocytes, and vascular endothelial cells.\u003cbr\u003e\nFunction: After C5a binding, the CD88 signal results in chemotaxis, granule enzyme release and superoxide anion production.\u003cbr\u003e\nInteraction: CD88 is coupled to heterotrimeric G proteins such as Gi, The signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules.\u003cbr\u003e\nBioactivity: Chemotaxis, degranulation.\u003cbr\u003e\nCell Type: Dendritic cells, Endothelial cells, Eosinophils, Monocytes, Neutrophils\u003cbr\u003e\nBiology Area: Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Schraufstatter IU, et al. 2009. J. Immunol. 182:3827. 2. Ippel JH, et al. 2009. J. Biol. Chem. 284:12363. 3. Monk PN, et al. 2007. Br. J. Pharmacol. 152:429. 4. Griffin RS, et al. 2007. J. Neurosci. 27:8699. 5. Lee H, et al. 2006. Nat. Biotechnol. 24:1279. 6. Buck E and Wells JA, et al. 2005. P. Natl. Acad. Sci. USA 102:2719.\u003cbr\u003e\nGene ID: 12273\u003cbr\u003e\nUniProt: View information about CD88 on UniProt.org\u003cbr\u003e\nClone: 20\/70\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: C5a ligand, C5a anaphylatoxin receptor, C5AR, C5R1, C5AR1\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860864585897,"sku":"135813","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-135813","provider":"Iright","version":"1.0","type":"link"}