{"product_id":"biolegend-135906","title":"Biolegend, 135906, PE anti-mouse CD140a Antibody, 200μg","description":"\u003cp\u003ePlatelet-derived growth factor receptor-α (PDGFR-α), CD140a, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs: PDGF-AA, PDGF-AB, and PDGF-BB. PDGFRa is a receptor tyrosine kinase that forms homodimers or heterodimers on the surface upon ligand binding and phosphorylates substrates. PDGFRs consist of either homodimers of α\/α, β\/β, or heterodimers of α\/β. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. Their ligand, PDGF, is a mitogen for connective tissue and glial cells. CD140a is expressed on embryonic tissues and mesenchymal-derived cells of adult mice. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes, and neutrophils.\u003cbr\u003e\n200μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse PDGFR-α-hIgG1 recombinant fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported (for relevant formats) applications include: Western Blot, blocking function2, and immunohistochemical staining of paraffin and frozen sections. The LEAF™ purified antibody is recommended for functional assays.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Takakura N, et al. 1996. J. Invest. Dermatol. 107:770. Liao C, et al. 2010. J. Clin. Invest. 120:242. (Block) Chen H, et al. 2015. ASN Neuro 8:7. PubMed. Miyawaki T, et al. 2004. J Neurosci. 24(37): 8124-34. (IHC-F)\u003cbr\u003e\nProduct Citations: Gabitova-Cornell L, et al. 2020. Cancer Cell. 38(4):567-583.e11. PubMed Vercauteren Drubbel A, et al. 2021. Cell Stem Cell. . PubMed Zelic M, et al. 2021. Cell Reports. 35(6):109112. PubMed Stoupa A, et al. 2018. EMBO Mol Med. 10:. PubMed Contreras O, et al. 2023. Front Cell Dev Biol. 11:1017660. PubMed He T, et al. 2023. iScience. 26:106289. PubMed Buechler MB, et al. 2021. Nature. 593:575. PubMed Frondelli MJ, et al. 2022. J Neurosci Res. 100:578. PubMed Malolina EA, et al. 2022. Mol Reprod Dev. 89:243. PubMed Poh AR, et al. 2022. Cell Rep. 41:111479. PubMed Wagner G, et al. 2017. Sci Rep. 7:40881. PubMed Huang Z et al. 2017. Cell metabolism. 26(3):493-508 . PubMed Salzer MC et al. 2018. Cell. 175(6):1575-1590 . PubMed Chen M, et al. 2020. Sci Adv. 6:eaax9605. PubMed Wu R, et al. 2019. J Cell Mol Med. 24:1684. PubMed Biffi G, et al. 2018. Cancer Discov. 2:282. PubMed Cardot-Ruffino V, et al. 2020. Genesis. 58:e23359. PubMed Lu A, et al. 2020. Sci Rep. 10:7075. PubMed Joshi PA, et al. 2019. Nat Commun. 10:1760. PubMed Jakob L, et al. 2021. Int J Mol Sci. 22:. PubMed Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed Diekmann U, et al. 2019. Sci Rep. 9:996. PubMed Frondelli MJ, et al. 2021. Neurotrauma Rep. 2:285. PubMed Ito K, et al. 2014. J Biol Chem. 289:29892. PubMed Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed Cardot-Ruffino V, et al. 2020. Sci Rep. 10:3880. PubMed Runyan CE, et al. 2020. Aging Cell. 19:00. PubMed Xi W, et al. 2021. Stem Cell Res Ther. 167:12. PubMed Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed Frech S, et al. 2021. J Invest Dermatol. . PubMed Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed Nawaz A, et al. 2018. Sci Rep. 6:37. PubMed Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed Fung CW, et al. 2022. Cell Discov. 8:61. PubMed Haider N, et al. 2020. Cell Commun Signal. 18:26. PubMed Shibamoto M, et al. 2019. Int Heart J. 60:944. PubMed Kim DK, et al. 2022. Nat Commun. 13:6292. PubMed Buffolo M, et al. 2019. Cell Rep. 29:270. PubMed Steele NG, et al. 2021. Clin Cancer Res. 27:2023. PubMed Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed Konishi S, et al. 2022. STAR Protoc. 3:101447. PubMed Rai S, et al. 2022. Nat Commun. 13:5346. PubMed Liu J, et al. 2020. Cell Mol Gastroenterol Hepatol. . PubMed Hara T, et al. 2021. Cancer Cell. 39(6):779-792.e11. PubMed Chen Y, et al. 2021. Cancer Cell. 39(4):548-565.e6. PubMed Kwon H, Kim S 2016. Cell Death Dis. 7:22285. PubMed Burl RB et al. 2018. Cell metabolism. 28(2):300-309 . PubMed Maller O, et al. 2020. Nat Mater. 20:548. PubMed Mahmoudi S, et al. 2019. Nature. 574:553. PubMed Wang Y, et al. 2020. Cell Reports. 31(5):107600. PubMed Velletri T, et al. 2020. Cell Death Differ. . PubMed Bravo JI, et al. 2021. STAR Protocols. 2(2):100406. PubMed Thomson CA, et al. 2018. J Immunol. 201:215. PubMed Ferrari N, et al. 2019. Nat Commun. 10:130. PubMed Velloso FJ, et al. 2022. STAR Protoc. 3:101065. PubMed\u003cbr\u003e\nRRID: AB_1953268 (BioLegend Cat. No. 135905) AB_1953269 (BioLegend Cat. No. 135906)\u003cbr\u003e\nStructure: Alpha chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.\u003cbr\u003e\nDistribution: Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.\u003cbr\u003e\nFunction: Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.\u003cbr\u003e\nLigand\/Receptor: PDGFs\u003cbr\u003e\nCell Type: Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Mukouyama YS, et al. 2006. Proc Natl Acad Sci USA. 103(5):1551 2. Miyawaki T, et al. 2004. J Neurosci. 24(37):8124 3. Takakura N, et al. 1997. J Histochem Cytochem. 45(6):883\u003cbr\u003e\nGene ID: 18595\u003cbr\u003e\nUniProt: View information about CD140a on UniProt.org\u003cbr\u003e\nClone: APA5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PDGF receptor-α, PDGFR-α\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862540538025,"sku":"135906","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-135906","provider":"Iright","version":"1.0","type":"link"}