{"product_id":"biolegend-135908","title":"Biolegend, 135908, APC anti-mouse CD140a Antibody, 100μg","description":"\u003cp\u003ePlatelet-derived growth factor receptor-α (PDGFR-α), CD140a, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs: PDGF-AA, PDGF-AB, and PDGF-BB. PDGFRa is a receptor tyrosine kinase that forms homodimers or heterodimers on the surface upon ligand binding and phosphorylates substrates. PDGFRs consist of either homodimers of α\/α, β\/β, or heterodimers of α\/β. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. Their ligand, PDGF, is a mitogen for connective tissue and glial cells. CD140a is expressed on embryonic tissues and mesenchymal-derived cells of adult mice. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes, and neutrophils.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse PDGFR-α-hIgG1 recombinant fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported (for relevant formats) applications include: Western Blot, blocking function2, and immunohistochemical staining of paraffin and frozen sections. The LEAF™ purified antibody is recommended for functional assays.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Takakura N, et al. 1996. J. Invest. Dermatol. 107:770. Liao C, et al. 2010. J. Clin. Invest. 120:242. (Block) Chen H, et al. 2015. ASN Neuro 8:7. PubMed. Miyawaki T, et al. 2004. J Neurosci. 24(37): 8124-34. (IHC-F)\u003cbr\u003e\nProduct Citations: Jadhav U et al. 2017. Cell stem cell. 21(1):65-77 . PubMed Korsunsky I, et al. 2022. Med. 3:481. PubMed Akasaka H, et al. 2023. JCI Insight. 8:. PubMed Wei Y, et al. 2021. Elife. 10: . PubMed Vasanthakumar A, et al. 2020. Nature. 579:581. PubMed Hattori Y, et al. 2022. J Neurosci. 42:362. PubMed Agarwalla P, et al. 2022. Nat Biotechnol. 40:1250. PubMed Sastourné-Arrey Q, et al. 2023. Nat Commun. 14:80. PubMed Corna G, et al. 2016. J Immunol. 197: 1914 - 1925. PubMed Wietecha MS, et al. 2020. Nat Commun. 2.266666667. PubMed Wang W, et al. 2019. Cell Metab. 30:174. PubMed Chen J, et al. 2021. Sci Adv. 7:. PubMed Vazquez-Armendariz AI, et al. 2021. STAR Protocols. 2(2):100594. PubMed Jard T, et al. 2020. Cell Stem Cell. 27(4):646-662.e7. PubMed Zhang X, et al. 2020. Cell Reports. 28(3):792-803.e4.. PubMed Nawaz A, et al. 2022. Nat Commun. 13:7058. PubMed Paris AJ, et al. 2020. Nat Cell Biol. 1.747916667. PubMed Chen YT, et al. 2021. Nat Commun. 12:6845. PubMed Ghelman J, et al. 2021. Glia. 69(11):2699-2716. PubMed Wang B,et al. 2017. Cell Discov.. 10.1038\/celldisc.2017.36. PubMed Gui J, et al. 2020. Nat Cancer. 1:603. PubMed Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed Bahar Halpern K, et al. 2020. Nat Commun. 11:1936. PubMed Reddy A, et al. 2020. Cell. 183:62. PubMed Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed Shook BA, et al. 2020. Cell Stem Cell. 26(6):880-895. PubMed Progatzky F, et al. 2021. Nature. 599:125. PubMed Ko T, et al. 2022. Nat Commun. 13:3275. PubMed Rebekka K Schneider et al. 2017. Cell stem cell. 20(6):785-800 . PubMed Hepler C et al. 2018. eLife. 7 pii: e39636. PubMed Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed Zhao T et al. 2018. Cell stem cell. 23(1):31-45 . PubMed Murakami Y, et al. 2019. Sci Rep. 1.188888889. PubMed Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed Jing Y, et al. 2020. Cancer Gene Ther. 27:910. PubMed Hogarth MW, et al. 2019. Nat Commun. 10:2430. PubMed Avraham S, et al. 2019. Oncogene. 38:3812. PubMed Wang QA et al. 2018. Cell metabolism. 28(2):282-288 . PubMed Shi X, et al. 2022. Clin Transl Med. 12:e711. PubMed Nowlan B, et al. 2019. Haematologica. 105:71. PubMed Hu Y, et al. 2021. EMBO Rep. 22:e52481. PubMed Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed Wang W, et al. 2014. Proc Natl Acad Sci U S A. 111:14466. PubMed Kurowska-Stolarska M, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31132-0. PubMed Moiseenko A, et al. 2020. Cell Rep. 33:108549. PubMed Lu Z, et al. 2021. J Clin Invest. 131:. PubMed Grainger N, et al. 2020. J Physiol. 598:3283. PubMed Cangkrama M, et al. 2020. EMBO Mol Med. 12:e11466. PubMed Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed Chandrakanthan V, et al. 2016. Proc Natl Acad Sci U S A. 113: 2306-2315. PubMed Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed Pinho AV, et al. 2018. Nat Commun. 9:5083. PubMed Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed Wong PP, et al. 2020. Cell. 181(6):1346-1363.e21. PubMed\u003cbr\u003e\nRRID: AB_2043969 (BioLegend Cat. No. 135907) AB_2043970 (BioLegend Cat. No. 135908)\u003cbr\u003e\nStructure: Alpha chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.\u003cbr\u003e\nDistribution: Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.\u003cbr\u003e\nFunction: Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.\u003cbr\u003e\nLigand\/Receptor: PDGFs\u003cbr\u003e\nCell Type: Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Mukouyama YS, et al. 2006. Proc Natl Acad Sci USA. 103(5):1551 2. Miyawaki T, et al. 2004. J Neurosci. 24(37):8124 3. Takakura N, et al. 1997. J Histochem Cytochem. 45(6):883\u003cbr\u003e\nGene ID: 18595\u003cbr\u003e\nUniProt: View information about CD140a on UniProt.org\u003cbr\u003e\nClone: APA5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PDGF receptor-α, PDGFR-α\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862540439721,"sku":"135908","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-135908","provider":"Iright","version":"1.0","type":"link"}