{"product_id":"biolegend-135910","title":"Biolegend, 135910, Biotin anti-mouse CD140a Antibody, 100μg","description":"\u003cp\u003ePlatelet-derived growth factor receptor-α (PDGFR-α), CD140a, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs: PDGF-AA, PDGF-AB, and PDGF-BB. PDGFRa is a receptor tyrosine kinase that forms homodimers or heterodimers on the surface upon ligand binding and phosphorylates substrates. PDGFRs consist of either homodimers of α\/α, β\/β, or heterodimers of α\/β. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. Their ligand, PDGF, is a mitogen for connective tissue and glial cells. CD140a is expressed on embryonic tissues and mesenchymal-derived cells of adult mice. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes, and neutrophils.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse PDGFR-α-hIgG1 recombinant fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported (for relevant formats) applications include: Western Blot, blocking function2, and immunohistochemical staining of paraffin and frozen sections. The LEAF™ purified antibody is recommended for functional assays.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Takakura N, et al. 1996. J. Invest. Dermatol. 107:770. Liao C, et al. 2010. J. Clin. Invest. 120:242. (Block) Chen H, et al. 2015. ASN Neuro 8:7. PubMed. Miyawaki T, et al. 2004. J Neurosci. 24(37): 8124-34. (IHC-F)\u003cbr\u003e\nProduct Citations: Ye B, et al. 2022. Cell Mol Immunol. 19:619. PubMed Helms EJ, et al. 2021. Cancer Discov. Online ahead of print. PubMed Larsen SB, et al. 2021. Cell Stem Cell. 28:1758. PubMed Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed Adam RC et al. 2018. Cell stem cell. 22(3):398-413 . PubMed Horiguchi H, et al. 2019. Genes Dev. 33:1641. PubMed Laurin M, et al. 2019. Elife. 8. PubMed Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed Kantelhardt S, et al. 2010. PLoS One. 5:e11323. PubMed Thomson CA, et al. 2018. J Immunol. 201:215. PubMed\u003cbr\u003e\nRRID: AB_2043973 (BioLegend Cat. No. 135909) AB_2043974 (BioLegend Cat. No. 135910)\u003cbr\u003e\nStructure: Alpha chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.\u003cbr\u003e\nDistribution: Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.\u003cbr\u003e\nFunction: Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.\u003cbr\u003e\nLigand\/Receptor: PDGFs\u003cbr\u003e\nCell Type: Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Mukouyama YS, et al. 2006. Proc Natl Acad Sci USA. 103(5):1551 2. Miyawaki T, et al. 2004. J Neurosci. 24(37):8124 3. Takakura N, et al. 1997. J Histochem Cytochem. 45(6):883\u003cbr\u003e\nGene ID: 18595\u003cbr\u003e\nUniProt: View information about CD140a on UniProt.org\u003cbr\u003e\nClone: APA5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PDGF receptor-α, PDGFR-α\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862540341417,"sku":"135910","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-135910","provider":"Iright","version":"1.0","type":"link"}