{"product_id":"biolegend-135923","title":"Biolegend, 135923, Brilliant Violet 421™ anti-mouse CD140a Antibody, 50μg","description":"\u003cp\u003ePlatelet-derived growth factor receptor-α (PDGFR-α), CD140a, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs: PDGF-AA, PDGF-AB, and PDGF-BB. PDGFRa is a receptor tyrosine kinase that forms homodimers or heterodimers on the surface upon ligand binding and phosphorylates substrates. PDGFRs consist of either homodimers of α\/α, β\/β, or heterodimers of α\/β. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. Their ligand, PDGF, is a mitogen for connective tissue and glial cells. CD140a is expressed on embryonic tissues and mesenchymal-derived cells of adult mice. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes, and neutrophils.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse PDGFR-α-hIgG1 recombinant fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported (for relevant formats) applications include: Western Blot, blocking function2, and immunohistochemical staining of paraffin and frozen sections. The LEAF™ purified antibody is recommended for functional assays.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Takakura N, et al. 1996. J. Invest. Dermatol. 107:770. Liao C, et al. 2010. J. Clin. Invest. 120:242. (Block) Chen H, et al. 2015. ASN Neuro 8:7. PubMed. Miyawaki T, et al. 2004. J Neurosci. 24(37): 8124-34. (IHC-F)\u003cbr\u003e\nProduct Citations: Hattori Y, et al. 2022. J Neurosci. 42:362. PubMed\u003cbr\u003e\nRRID: AB_2814036 (BioLegend Cat. No. 135923)\u003cbr\u003e\nStructure: Alpha chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.\u003cbr\u003e\nDistribution: Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.\u003cbr\u003e\nFunction: Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.\u003cbr\u003e\nLigand\/Receptor: PDGFs\u003cbr\u003e\nCell Type: Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Mukouyama YS, et al. 2006. Proc Natl Acad Sci USA. 103(5):1551 2. Miyawaki T, et al. 2004. J Neurosci. 24(37):8124 3. Takakura N, et al. 1997. J Histochem Cytochem. 45(6):883\u003cbr\u003e\nGene ID: 18595\u003cbr\u003e\nUniProt: View information about CD140a on UniProt.org\u003cbr\u003e\nClone: APA5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PDGF receptor-α, PDGFR-α\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862540996777,"sku":"135923","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-135923","provider":"Iright","version":"1.0","type":"link"}