{"product_id":"biolegend-136412","title":"Biolegend, 136412, PerCP\/Cyanine5.5 anti-mouse CD309 (VEGFR2, Flk-1) Antibody, 100μg","description":"\u003cp\u003eCD309 is also known as vascular endothelial growth factor receptor 2 (VEGFR2) and fetal liver kinase-1 (Flk-1). CD309 is a member of the tyrosine protein kinase family that contains a single pass transmembrane receptor with a protein kinase domain and seven immunoglobulin-like domains in the extracellular region. CD309 is expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle. It's a receptor for VEGF or VEGFC, and plays an important role in the development of vascular endothelial cells, hematopoietic cells, and vascular permeability.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Murine Flk1 fused to hIgG Fc\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Avas12 recognizes a different epitope than clone 89B3A5. Additional reported applications (for the relevant formats) include: Western blotting1 and immunohistochemical staining of paraformaldehyde-fixed frozen sections2.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kataoka H, et al. 1997. Dev. Growth Differ. 39:729. (WB) Ishitobi H, et al. 2010. Exp. Anim. 59:615. (IHC-F)\u003cbr\u003e\nRRID: AB_2132488 (BioLegend Cat. No. 136412)\u003cbr\u003e\nStructure: A receptor protein tyrosine kinase closely related to CD117 (c-kit) and CD140a (PDGF receptor α chain) of the immunoglobulin superfamily\u003cbr\u003e\nDistribution: Expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle\u003cbr\u003e\nFunction: Plays an important role in the development of vascular endothelial cells, hematopoietic cells, and vascular permeability\u003cbr\u003e\nLigand\/Receptor: Vascular endothelial growth factor (VEGF)\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Biology, Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Kaburn N, et al. 1997. Development. 124:2039 2. Patterson C, et al. 1995. J. Bio. Chem. 270:23111 3. Nishikawa SI, et al. 1998. Immunity 8 (6):761 4. Shalaby F, et al. 1997. Cell. 89 (6):981\u003cbr\u003e\nGene ID: 16542\u003cbr\u003e\nUniProt: View information about CD309 on UniProt.org\u003cbr\u003e\nClone: Avas12\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Fetal liver kinase 1 (Flk-1), VEGF receptor-2, VEGF-R2, Ly-73, KDR\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862544601257,"sku":"136412","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-136412","provider":"Iright","version":"1.0","type":"link"}