{"product_id":"biolegend-137603","title":"Biolegend, 137603, PE anti-mouse CD335 (NKp46) Antibody, 25μg","description":"\u003cp\u003eCD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: NKP46-IgG1 Fc fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2 and in vitro activation of NK cells1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ) Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ) Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) PubMed\u003cbr\u003e\nProduct Citations: Palacios-Arreola M, et al. 2017. Sci Rep. 10.1038\/s41598-017-10135-1. PubMed Vogt M, et al. 2011. J Virol. 85:11567. PubMed Li T, et al. 2023. Nat Commun. 14:2498. PubMed Hattori K, et al. 2023. Biomed Rep. 18:1. PubMed Fu R, et al. 2020. Sci Rep. 10:1455. PubMed Li Y, et al. 2014. PLoS One. 9:86927. PubMed Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed Cluff E, et al. 2022. Cancer Immunol Immunother. . PubMed Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed Victorino F, et al. 2015. J Immunol. 195: 4973 - 4985. PubMed Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed Best SA, et al. 2018. Cell Metab. 27:935. PubMed Li J, et al. 2020. Cancer Discov. . PubMed Schaffert A, et al. 2020. Front Immunol. 10:3024. PubMed Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed Li J, et al. 2020. Cancer Immunol Res. 0.529166667. PubMed Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed Progatzky F, et al. 2021. Nature. 599:125. PubMed Ruiz–Manzano RA, et al. 2020. Frontiers in Oncology. 0.927777778. PubMed Ye X, et al. 2021. Cancer Biol Med. Online ahead of print. PubMed Yuan X, et al. 2017. Elife. 6:e29540. PubMed Kang YH, et al. 2019. Nat Commun. 10:912. PubMed Zhao L,et al. 2017. Nat Commun. . 10.1038\/s41467-017-01181-4. PubMed Sheppard S, et al. 2021. Cell Reports. 35(9):109210. PubMed Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed Teng F, et al. 2021. Cell Rep. 37:110051. PubMed Liu CF, et al. 2017. Front Immunol. 1.445833333. PubMed Guo S, et al. 2021. Frontiers in Immunology. 11:614667. PubMed\u003cbr\u003e\nRRID: AB_10552741 (BioLegend Cat. No. 137603) AB_2235755 (BioLegend Cat. No. 137604)\u003cbr\u003e\nStructure: Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains\u003cbr\u003e\nDistribution: Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells\u003cbr\u003e\nFunction: NK cells activation\u003cbr\u003e\nLigand\/Receptor: Viral hemagglutinins, heparan sulfate proteoglycans\u003cbr\u003e\nCell Type: NK cells, NKT cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60. 2. Caligiuri MA. 2008. Blood 112:461. 3. Colonna M. 2009. Immunity 31:15.\u003cbr\u003e\nGene ID: 17086\u003cbr\u003e\nUniProt: View information about CD335 on UniProt.org\u003cbr\u003e\nClone: 29A1.4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: NKp46, NCR1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866544459945,"sku":"137603","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-137603","provider":"Iright","version":"1.0","type":"link"}